You are viewing an incomplete version of our website. Please click to reload the website as full version.

AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 551-663)

Details for Product anti-AKAP8 Antibody No. ABIN393705, Supplier: Log in to see
Antigen
  • MGC108502
  • AKAP8
  • AKAP 95
  • AKAP-8
  • AKAP-95
  • AKAP95
  • 1200016A02Rik
  • AA673585
  • AU015639
  • Akap95
Epitope
AA 551-663
12
10
6
6
4
3
2
2
1
1
1
1
1
1
Reactivity
Human
84
33
32
9
5
1
1
Host
Mouse
54
16
15
Clonality (Clone)
Monoclonal ()
Conjugate
This AKAP8 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
67
28
25
16
12
11
7
5
3
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D4
Isotype IgG2a kappa
Specificity AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP8 (AKAP8 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 76108 DA
Gene ID 12406
NCBI Accession NP_005849
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 551-663) (ABIN393705) Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 551-663) (ABIN393705) anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 551-663) antibody (Image 2)
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Kamada, Kikkawa, Tsujimoto et al.: "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9469-77, 2005 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).