anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 551-663)

Details for Product anti-AKAP8 Antibody No. ABIN393705
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Synonyms MGC108502, AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95, 1200016A02Rik, AA673585, AU015639, Akap95
AA 551-663
(13), (9), (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
(85), (34), (32), (9), (5), (1), (1)
(55), (16), (15)
Clonality (Clone)
Monoclonal ()
This AKAP8 antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(70), (32), (28), (17), (11), (10), (6), (5), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Availability Will be delivered in 8 to 11 Business Days
Immunogen AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D4
Isotype IgG2a kappa
Specificity AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP8 (AKAP8 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 76108 DA
Gene ID 12406
NCBI Accession NP_005849
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 551-663) (ABIN393705) Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-AKAP8 antibody (A Kinase (PRKA) Anchor Protein 8) (AA 551-663) (ABIN393705) anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 551-663) antibody (Image 2)
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Kamada, Kikkawa, Tsujimoto et al.: "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9469-77, 2005 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Catalog No. ABIN393705
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