A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 551-663) antibody

Details for Product No. ABIN393705
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Antigen
Synonyms MGC108502, AKAP8, AKAP 95, AKAP-8, AKAP-95, AKAP95, 1200016A02Rik, AA673585, AU015639, Akap95
Epitope
AA 551-663
(8), (4), (3), (3), (2), (2), (1), (1)
Reactivity
Human
(63), (31), (29), (16), (12), (12), (2)
Host
Mouse
(49), (10), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(50), (25), (16), (16), (11), (11), (10), (6), (4), (2)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393705
450.00 $
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Immunogen AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D4
Isotype IgG2a kappa
Specificity AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP8
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 76108 DA
Gene ID 12406
NCBI Accession NP_005849
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 551-663) antibody Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody (M01), clone 3D4. Lane 1: AKAP8 transfected lysate(76.2 KDa). Lane 2: Non-transfected lysate.
anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 551-663) antibody (2) anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) (AA 551-663) antibody (Image 2)
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Kamada, Kikkawa, Tsujimoto et al.: "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9469-77, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Validation Images
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