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Details for Product No. ABIN393705

A Kinase (PRKA) Anchor Protein 8 (AKAP8) antibody

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Antigen
Synonyms AKAP 95, AKAP-8, AKAP-95, AKAP95, AA673585, AU015639, 1200016A02Rik, Akap95, MGC108502, AKAP8
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393705
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Immunogen AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D4
Isotype IgG2a kappa
Specificity AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP8
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 76108 DA
Gene ID 12406
UniProt NP_005849
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence
Comment

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Kamada, Kikkawa, Tsujimoto et al.: "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9469-77, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Alternatives for antigen "A Kinase (PRKA) Anchor Protein 8 (AKAP8)", type "Antibodies"
Hosts (35), (10), (6)
Reactivities (48), (24), (23), (16), (12), (12), (2)
Applications (38), (17), (15), (10), (8), (8), (5), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (3), (3), (3), (2)
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