A Kinase (PRKA) Anchor Protein 8 (AKAP8) antibody

Antigen: A Kinase (PRKA) Anchor Protein 8 (AKAP8)

Synonyms: AKAP95, DKFZp586B1222, AA673585, AU015639, 1200016A02Rik, Akap95, AKAP8, MGC165917, MGC108502

Clonality: Monoclonal (3D4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393705

Additional Information

Alternative name: AKAP8

Gene ID: 10270

UniProt: NP_005849

Immunogen: AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3D4

Description: Other names: AKAP95, DKFZp586B1222 A kinase (PRKA) anchor protein 8

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AKAP8 (NP_005849, 551 a.a. ~ 663 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 76108 DA

Comments:

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to AKAP8 (AT1089a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody (M01), clone 3D4. Lane 1: AKAP8 transfected lysate(76.2 KDa). Lane 2: Non-transfected lysate.

anti-A Kinase (PRKA) Anchor Protein 8 (AKAP8) antibody (Image 2)

Publications

Product: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Kamada, Kikkawa, Tsujimoto et al.: "A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9469-77, 2005 (PubMed).

Beausoleil, Villén, Gerber et al.: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).