You are viewing an incomplete version of our website. Please click to reload the website as full version.

Ubiquitin-Conjugating Enzyme E2A (ube2a) (AA 1-153) antibody

Details for Product No. ABIN393708, Supplier: Log in to see
Antigen
  • BHR6A
  • HR6A
  • hhr6a
  • rad6a
  • ubc2
  • mp:zf637-2-000771
  • si:bz46j2.4
  • wu:fa01h11
  • HHR6A
  • MRXS30
  • MRXSN
  • RAD6A
  • UBC2
  • Mhr6a
Epitope
AA 1-153
4
3
3
2
2
Reactivity
Human
45
20
20
Host
Mouse
35
10
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
29
11
11
9
9
6
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen UBE2A (AAH10175, 1 a.a. ~ 153 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D7-E2
Isotype IgG1 kappa
Specificity UBE2A (AAH10175, 1 a.a. ~ 153 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2A (ube2a Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17315 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Nascimento, Otto, de Brouwer et al.: "UBE2A, which encodes a ubiquitin-conjugating enzyme, is mutated in a novel X-linked mental retardation syndrome." in: American journal of human genetics, Vol. 79, Issue 3, pp. 549-55, 2006 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Kunapuli, Somerville, Still et al.: "ZNF198 protein, involved in rearrangement in myeloproliferative disease, forms complexes with the DNA repair-associated HHR6A/6B and RAD18 proteins." in: Oncogene, Vol. 22, Issue 22, pp. 3417-23, 2003 (PubMed).

Lyakhovich, Shekhar: "Supramolecular complex formation between Rad6 and proteins of the p53 pathway during DNA damage-induced response." in: Molecular and cellular biology, Vol. 23, Issue 7, pp. 2463-75, 2003 (PubMed).

Suzumori, Burns, Yan et al.: "RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 550-5, 2003 (PubMed).