Ubiquitin-Conjugating Enzyme E2A (ube2a) antibody

Details for Product No. ABIN393708
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Antigen
Synonyms HHR6A, MRXS30, MRXSN, RAD6A, UBC2, HR6A, Mhr6a, BHR6A, hhr6a, rad6a, ubc2, mp:zf637-2-000771, si:bz46j2.4, wu:fa01h11
Reactivity
Human
(32), (16), (15), (12), (12)
Host
Mouse
(25), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(20), (12), (10), (6), (6), (5), (4), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393708
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen UBE2A (AAH10175, 1 a.a. ~ 153 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D7-E2
Isotype IgG1 kappa
Specificity UBE2A (AAH10175, 1 a.a. ~ 153 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17315 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Suzumori, Burns, Yan et al.: "RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 550-5, 2003 (PubMed).

Lyakhovich, Shekhar: "Supramolecular complex formation between Rad6 and proteins of the p53 pathway during DNA damage-induced response." in: Molecular and cellular biology, Vol. 23, Issue 7, pp. 2463-75, 2003 (PubMed).

Kunapuli, Somerville, Still et al.: "ZNF198 protein, involved in rearrangement in myeloproliferative disease, forms complexes with the DNA repair-associated HHR6A/6B and RAD18 proteins." in: Oncogene, Vol. 22, Issue 22, pp. 3417-23, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Nascimento, Otto, de Brouwer et al.: "UBE2A, which encodes a ubiquitin-conjugating enzyme, is mutated in a novel X-linked mental retardation syndrome." in: American journal of human genetics, Vol. 79, Issue 3, pp. 549-55, 2006 (PubMed).

Hosts (25), (8)
Reactivities (32), (16), (15), (12), (12)
Applications (20), (12), (10), (6), (6), (5), (4), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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