Ubiquitin-Conjugating Enzyme E2A (ube2a) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2A (ube2a)

Synonyms: UBC2, HHR6A, RAD6A, HR6A, Mhr6a, UBE2A, BHR6A, MGC127256, MGC68540, ubc2, hhr6a, rad6a, MGC69378, MGC64109, wu:fa01h11, si:bz46j2.4, mp:zf637-2-000771

Clonality: Monoclonal (3D7-E2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393708

Additional Information

Alternative name: UBE2A

Gene ID: 7319

Immunogen: UBE2A (AAH10175, 1 a.a. ~ 153 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D7-E2

Description: Other names: HHR6A, RAD6A, UBC2 ubiquitin-conjugating enzyme E2A (RAD6 homolog)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UBE2A (AAH10175, 1 a.a. ~ 153 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 17315 DA

Comments:

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Suzumori, Burns, Yan et al.: "RFPL4 interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 550-5, 2003 (PubMed).

Lyakhovich, Shekhar: "Supramolecular complex formation between Rad6 and proteins of the p53 pathway during DNA damage-induced response." in: Molecular and cellular biology, Vol. 23, Issue 7, pp. 2463-75, 2003 (PubMed).

Kunapuli, Somerville, Still et al.: "ZNF198 protein, involved in rearrangement in myeloproliferative disease, forms complexes with the DNA repair-associated HHR6A/6B and RAD18 proteins." in: Oncogene, Vol. 22, Issue 22, pp. 3417-23, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Nascimento, Otto, de Brouwer et al.: "UBE2A, which encodes a ubiquitin-conjugating enzyme, is mutated in a novel X-linked mental retardation syndrome." in: American journal of human genetics, Vol. 79, Issue 3, pp. 549-55, 2006 (PubMed).