X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5)

Synonyms: XRCC5, MGC179425, DKFZp469K071

Clonality: Monoclonal (3D8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393709

Additional Information

Alternative name: XRCC5

Gene ID: 7520

Immunogen: XRCC5 (AAH19027, 1 a.a. ~ 733 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D8

Description: Other names: KARP1, KARP1, KU80, KUB2, Ku86, NFIV X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining, Ku autoantigen, 80kDa)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: XRCC5 (AAH19027, 1 a.a. ~ 733 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 82705 DA

Comments:

Background: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

XRCC5 monoclonal antibody (M02), clone 3D8 Western Blot analysis of XRCC5 expression in A-431 ( Cat # L015V1 ). Immunoperoxidase of monoclonal antibody to XRCC5 ( Cat# AT4553a ) on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]

anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5) antibody (Image 2)

Publications

Product: Monsees, Kraft, Chanock et al.: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010 (PubMed).

Briggs, Goldstein, McCauley et al.: "Variation within DNA repair pathway genes and risk of multiple sclerosis." in: American journal of epidemiology, Vol. 172, Issue 2, pp. 217-24, 2010 (PubMed).

Liu, Shete, Wang et al.: "Gamma-radiation sensitivity and polymorphisms in RAD51L1 modulate glioma risk." in: Carcinogenesis, Vol. 31, Issue 10, pp. 1762-9, 2010 (PubMed).

Ho-Pun-Cheung, Assenat, Bascoul-Mollevi et al.: "A large-scale candidate gene approach identifies SNPs in SOD2 and IL13 as predictive markers of response to preoperative chemoradiation in rectal cancer." in: The pharmacogenomics journal, Vol. 11, Issue 6, pp. 437-43, 2011 (PubMed).

Gomes, Silva, Azevedo et al.: "The role of common variants of non-homologous end-joining repair genes XRCC4, LIG4 and Ku80 in thyroid cancer risk." in: Oncology reports, Vol. 24, Issue 4, pp. 1079-85, 2010 (PubMed).