WD Repeat Domain 77 (WDR77) (AA 1-343) antibody

Details for Product No. ABIN393715
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Antigen
Synonyms MEP-50, mep50, valois, WDR77, MEP50, Nbla10071, RP11-552M11.3, p44, p44/Mep50, 2610003I18Rik, 2610312E17Rik, C79984, p44/MEP50, RGD1310479
Epitope
AA 1-343
(8), (3), (3), (2), (2), (1), (1)
Reactivity
Human
(26), (6), (6), (2), (1), (1)
Host
Mouse
(14), (13)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(23), (15), (8), (6), (5), (4), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393715
450.00 $
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Immunogen WDR77 (AAH01679, 1 a.a. ~ 343 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3F10
Isotype IgG1 kappa
Specificity WDR77 (AAH01679, 1 a.a. ~ 343 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name WDR77
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 36724 DA
Gene ID 12406
Research Area Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-WD Repeat Domain 77 (WDR77) (AA 1-343) antibody WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa on formalin-fixed paraffin-embedded human tonsil (antibody concentration 3 µg/mL).
anti-WD Repeat Domain 77 (WDR77) (AA 1-343) antibody (2) anti-WD Repeat Domain 77 (WDR77) (AA 1-343) antibody (Image 2)
Background publications Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ouararhni, Hadj-Slimane, Ait-Si-Ali et al.: "The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity." in: Genes & development, Vol. 20, Issue 23, pp. 3324-36, 2006 (PubMed).

Liang, Wang, Chiriboga et al.: "The expression and function of androgen receptor coactivator p44 and protein arginine methyltransferase 5 in the developing testis and testicular tumors." in: The Journal of urology, Vol. 177, Issue 5, pp. 1918-22, 2007 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Peng, Chen, Melamed et al.: "Distinct nuclear and cytoplasmic functions of androgen receptor cofactor p44 and association with androgen-independent prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 13, pp. 5236-41, 2008 (PubMed).

Validation Images
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