WD Repeat Domain 77 (WDR77) antibody

Antigen: WD Repeat Domain 77 (WDR77)

Synonyms: p44, MEP50, MGC2722, HKMT1069, Nbla10071, RP11-552M11.3, C79984, p44/MEP50, 2610003I18Rik, 2610312E17Rik, RGD1310479, MGC81050, WDR77, DKFZp459I107

Clonality: Monoclonal (3F10)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393715

Additional Information

Alternative name: WDR77

Gene ID: 79084

Immunogen: WDR77 (AAH01679, 1 a.a. ~ 343 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3F10

Description: Other names: HKMT1069, MEP50, MGC2722 WD repeat domain 77

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WDR77 (AAH01679, 1 a.a. ~ 343 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 36724 DA

Comments:

Background: WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to WDR77 ( Cat # AT4536a ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml]

anti-WD Repeat Domain 77 (WDR77) antibody (Image 2)

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Ouararhni, Hadj-Slimane, Ait-Si-Ali et al.: "The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity." in: Genes & development, Vol. 20, Issue 23, pp. 3324-36, 2006 (PubMed).

Liang, Wang, Chiriboga et al.: "The expression and function of androgen receptor coactivator p44 and protein arginine methyltransferase 5 in the developing testis and testicular tumors." in: The Journal of urology, Vol. 177, Issue 5, pp. 1918-22, 2007 (PubMed).

Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).

Peng, Chen, Melamed et al.: "Distinct nuclear and cytoplasmic functions of androgen receptor cofactor p44 and association with androgen-independent prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 13, pp. 5236-41, 2008 (PubMed).