BCAR3 antibody (Breast Cancer Anti-Estrogen Resistance 3) (AA 266-373)

Details for Product anti-BCAR3 Antibody No. ABIN393728, Supplier: Log in to see
Antigen
  • BCAR3
  • AI131758
  • AND-34
  • NSP2
  • SH2D3B
  • breast cancer anti-estrogen resistance 3
  • BCAR3
  • bcar3
  • LOC100219682
  • Bcar3
Epitope
AA 266-373
18
5
4
2
2
2
1
1
1
Reactivity
Human
33
22
8
3
2
1
1
Host
Mouse
27
15
4
Clonality (Clone)
Monoclonal ()
Conjugate
This BCAR3 antibody is un-conjugated
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
37
26
11
5
5
3
2
2
2
1
1
Options
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Immunogen BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G4
Isotype IgG2a kappa
Specificity BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BCAR3 (BCAR3 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 92566 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-BCAR3 antibody (Breast Cancer Anti-Estrogen Resistance 3) (AA 266-373) (ABIN393728) Detection limit for recombinant GST tagged BCAR3 is approximately 0.03 ng/mL as a cap...
Background publications Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Schuh, Guerrero, Schrecengost, Bouton: "BCAR3 regulates Src/p130 Cas association, Src kinase activity, and breast cancer adhesion signaling." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2309-17, 2010 (PubMed).

Talmud, Drenos, Shah, Shah, Palmen, Verzilli, Gaunt, Pallas, Lovering, Li, Casas, Sofat, Kumari, Rodriguez, Johnson, Newhouse, Dominiczak, Samani, Caulfield, Sever, Stanton, Shields, Padmanabhan et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. ..." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Makkinje, Near, Infusini, Vanden Borre, Bloom, Cai, Costello, Lerner: "AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern." in: Cellular signalling, Vol. 21, Issue 9, pp. 1423-35, 2009 (PubMed).

Oh, van Agthoven, Choi, Jeong, Chung, Kim, Jhun: "BCAR3 regulates EGF-induced DNA synthesis in normal human breast MCF-12A cells." in: Biochemical and biophysical research communications, Vol. 375, Issue 3, pp. 430-4, 2008 (PubMed).