|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) antibody
|Synonyms||AND-34, AI131758, BCAR3, MGC127863, bcar3, CAS, CAS1, CASS1, CRKAS, P130Cas, Cas, Crkas, AI385681, P130CAS, MGC68466, cas, p130cas, BCAR1, bcar1, fc17f11, MGC175192, wu:fc17f11|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: KIAA0554, NSP2, SH2D3B breast cancer anti-estrogen resistance|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||92566 DA|
Background: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged BCAR3 is approximately 0.03ng/ml as a capture antibody.|
Oh, van Agthoven, Choi et al.: "BCAR3 regulates EGF-induced DNA synthesis in normal human breast MCF-12A cells." in: Biochemical and biophysical research communications, Vol. 375, Issue 3, pp. 430-4, 2008 (PubMed).
Makkinje, Near, Infusini et al.: "AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern." in: Cellular signalling, Vol. 21, Issue 9, pp. 1423-35, 2009 (PubMed).
Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).
Schuh, Guerrero, Schrecengost et al.: "BCAR3 regulates Src/p130 Cas association, Src kinase activity, and breast cancer adhesion signaling." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2309-17, 2010 (PubMed).
Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).
|Hosts||Rabbit (8), Goat (3)|
|Reactivities||Human (9), Mouse (Murine) (2), Rat (Rattus) (2)|
|Applications||Western Blotting (WB) (8), ELISA (5), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1), Immunohistochemistry (IHC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|
|Epitopes||N-Term (5), C-Term (1)|