Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) antibody

Antigen: Breast Cancer Anti-Estrogen Resistance 3 (BCAR3)

Synonyms: AND-34, AI131758, BCAR3, MGC127863, bcar3, CAS, CAS1, CASS1, CRKAS, P130Cas, Cas, Crkas, AI385681, P130CAS, MGC68466, cas, p130cas, BCAR1, bcar1, fc17f11, MGC175192, wu:fc17f11

Clonality: Monoclonal (3G4)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393728

Additional Information

Alternative name: BCAR3

Gene ID: 8412

Immunogen: BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3G4

Description: Other names: KIAA0554, NSP2, SH2D3B breast cancer anti-estrogen resistance

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 92566 DA

Comments:

Background: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged BCAR3 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Oh, van Agthoven, Choi et al.: "BCAR3 regulates EGF-induced DNA synthesis in normal human breast MCF-12A cells." in: Biochemical and biophysical research communications, Vol. 375, Issue 3, pp. 430-4, 2008 (PubMed).

Makkinje, Near, Infusini et al.: "AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern." in: Cellular signalling, Vol. 21, Issue 9, pp. 1423-35, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Schuh, Guerrero, Schrecengost et al.: "BCAR3 regulates Src/p130 Cas association, Src kinase activity, and breast cancer adhesion signaling." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2309-17, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).