Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) antibody

Details for Product No. ABIN393728
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Antigen
Synonyms AND-34, AI131758, NSP2, SH2D3B, BCAR3, MGC68466, cas, Crkas, p130cas, BCAR1, fc17f11, wu:fc17f11, zgc:175192
Reactivity
Human
(25), (7), (6)
Host
Mouse
(19), (4), (4)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
(24), (18), (8), (4), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393728
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G4
Isotype IgG2a kappa
Specificity BCAR3 (AAH39895, 266 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BCAR3
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 92566 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) antibody Detection limit for recombinant GST tagged BCAR3 is approximately 0.03 ng/mL as a capture antibody.
Background publications Oh, van Agthoven, Choi et al.: "BCAR3 regulates EGF-induced DNA synthesis in normal human breast MCF-12A cells." in: Biochemical and biophysical research communications, Vol. 375, Issue 3, pp. 430-4, 2008 (PubMed).

Makkinje, Near, Infusini et al.: "AND-34/BCAR3 regulates adhesion-dependent p130Cas serine phosphorylation and breast cancer cell growth pattern." in: Cellular signalling, Vol. 21, Issue 9, pp. 1423-35, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Schuh, Guerrero, Schrecengost et al.: "BCAR3 regulates Src/p130 Cas association, Src kinase activity, and breast cancer adhesion signaling." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2309-17, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Hosts (19), (4), (4)
Reactivities (25), (7), (6)
Applications (24), (18), (8), (4), (3), (2), (1)
Epitopes (8), (2), (2)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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