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USP48 antibody (Ubiquitin Specific Peptidase 48) (AA 110-220)

Details for Product anti-USP48 Antibody No. ABIN393729, Supplier: Log in to see
Antigen
  • USP48
  • USP31
  • MGC135142
  • RAP1GA1
  • 2810449C13Rik
  • AI115503
  • BC021769
  • D330022K21Rik
  • Usp31
  • synUSP
  • zgc:112364
Epitope
AA 110-220
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human
29
14
14
6
4
2
1
Host
Mouse
24
5
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
23
14
14
2
1
1
Supplier
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Immunogen USP48 (NP_115612, 110 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G4
Isotype IgG1 kappa
Specificity USP48 (NP_115612, 110 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name USP48 (USP48 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 119032 DA
Gene ID 12406
NCBI Accession NP_115612
Research Area Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-USP48 antibody (Ubiquitin Specific Peptidase 48) (AA 110-220) (ABIN393729) USP48 monoclonal antibody (M01), clone 3G4 Western Blot analysis of USP48 expression ...
Immunohistochemistry (IHC) image for anti-USP48 antibody (Ubiquitin Specific Peptidase 48) (AA 110-220) (ABIN393729) anti-Ubiquitin Specific Peptidase 48 (USP48) (AA 110-220) antibody (Image 2)
Background publications Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Tzimas, Michailidou, Arsenakis et al.: "Human ubiquitin specific protease 31 is a deubiquitinating enzyme implicated in activation of nuclear factor-kappaB." in: Cellular signalling, Vol. 18, Issue 1, pp. 83-92, 2005 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).