Ubiquitin Specific Peptidase 48 (USP48) antibody

Antigen: Ubiquitin Specific Peptidase 48 (USP48)

Synonyms: USP31, RAP1GA1, MGC14879, MGC132556, DKFZp762M1713, Usp31, AI115503, BC021769, MGC25724, 2810449C13Rik, D330022K21Rik, synUSP, USP48, MGC135142, MGC112364, zgc:112364

Clonality: Monoclonal (3G4)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393729

Additional Information

Alternative name: USP48

Gene ID: 84196

UniProt: NP_115612

Immunogen: USP48 (NP_115612, 110 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3G4

Description: Other names: DKFZp762M1713, MGC14879, RAP1GA1, USP31 ubiquitin specific protease 48

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: USP48 (NP_115612, 110 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 119032 DA

Comments:

Background: This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

USP48 monoclonal antibody (M01), clone 3G4 Western Blot analysis of USP48 expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to USP48 ( Cat # AT4494a ) on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

anti-Ubiquitin Specific Peptidase 48 (USP48) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Tzimas, Michailidou, Arsenakis et al.: "Human ubiquitin specific protease 31 is a deubiquitinating enzyme implicated in activation of nuclear factor-kappaB." in: Cellular signalling, Vol. 18, Issue 1, pp. 83-92, 2005 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).