Adenylate Kinase 1 (AK1) antibody

Antigen: Adenylate Kinase 1 (AK1)

Synonyms: ADK-1, AK1, DAK1, bs34e10.y1, DmelCG17146, CG17146, MGC140285, ak5, MGC79778, ADENYLATE KINASE 1, ADK1, MLE2.3, MLE2_3, DKFZp459A1921, Ak-1, B430205N08Rik, MGC108678

Clonality: Monoclonal (3G8-1B11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393734

Additional Information

Alternative name: AK1

Gene ID: 203

Immunogen: AK1 (AAH01116, 1 a.a. ~ 195 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3G8-1B11

Description: Other names: adenylate kinase 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AK1 (AAH01116, 1 a.a. ~ 195 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 21635 DA

Comments:

Background: Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of to AK1 ( Cat# AT1079a ) on HeLa cell .

Publications

Product: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Topalis, Alvarez, Barral et al.: "Acyclic phosphonate nucleotides and human adenylate kinases: impact of a borano group on alpha-P position." in: Nucleosides, nucleotides & nucleic acids, Vol. 27, Issue 4, pp. 319-31, 2008 (PubMed).

Gloria-Bottini, Magrini, Pietropolli et al.: "Smoking and human reproduction: the effect of adenylate kinase genetic polymorphism." in: American journal of perinatology, Vol. 26, Issue 2, pp. 117-21, 2009 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia." in: European archives of psychiatry and clinical neuroscience, Vol. 259, Issue 3, pp. 151-63, 2009 (PubMed).