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SH3 Domain Containing Ring Finger 1 (SH3RF1) (AA 790-889) antibody

Details for Product No. ABIN393739
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Antigen
Synonyms SH3MD2, posh, xPOSH, rnf142, sh3md2, MGC135632, MGC189605, POSH, RNF142, 2200003J05Rik, Posh, R75531, Sh3md2, si:ch211-114c9.2, si:dkey-15j16.4, wu:fi42f11
Epitope
AA 790-889
(6), (3), (1), (1), (1), (1)
Reactivity
Human
(29), (17), (16), (13), (12), (12), (1)
Host
Mouse
(20), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(21), (12), (10), (4), (3)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393739
450.00 $
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Immunogen SH3MD2 (NP_065921, 790 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3H3
Isotype IgG2a kappa
Specificity SH3MD2 (NP_065921, 790 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SH3MD2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 93129 DA
Gene ID 12406
UniProt NP_065921
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) (AA 790-889) antibody SH3MD2 monoclonal antibody (M01), clone 3H3 Western Blot analysis of SH3MD2 expression in HeLa. Detection limit for recombinant GST tagged SH3MD2 is approximately 0.03 ng/mL as a capture antibody.
anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) (AA 790-889) antibody (2) anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) (AA 790-889) antibody (Image 2)
Background publications Kärkkäinen, Hiipakka, Wang et al.: "Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome." in: EMBO reports, Vol. 7, Issue 2, pp. 186-91, 2006 (PubMed).

Lyons, Thorburn, Ryan et al.: "Regulation of the Pro-apoptotic scaffolding protein POSH by Akt." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 21987-97, 2007 (PubMed).

Votteler, Iavnilovitch, Fingrut et al.: "Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release." in: BMC biochemistry, Vol. 10, pp. 12, 2009 (PubMed).

Lin, Yue, Pan et al.: "POSH stimulates the ubiquitination and the clathrin-independent endocytosis of ROMK1 channels." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29614-24, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Validation Images
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