SH3 Domain Containing Ring Finger 1 (SH3RF1) antibody

Details for Product No. ABIN393739
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Synonyms POSH, RNF142, SH3MD2, Posh, R75531, Sh3md2, 2200003J05Rik, posh, rnf142, sh3md2, xPOSH, MGC135632, MGC189605
(23), (17), (16), (13), (12), (12), (1)
(20), (4)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(14), (10), (9), (3), (3), (1)
Pubmed 5 references available
Catalog no. ABIN393739
Quantity 0.1 mg
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Immunogen SH3MD2 (NP_065921, 790 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3H3
Isotype IgG2a kappa
Specificity SH3MD2 (NP_065921, 790 a.a. ~ 889 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SH3MD2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 93129 DA
Gene ID 12406
UniProt NP_065921
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000

Background: This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) antibody SH3MD2 monoclonal antibody (M01), clone 3H3 Western Blot analysis of SH3MD2 expression in HeLa. Detection limit for recombinant GST tagged SH3MD2 is approximately 0.03 ng/mL as a capture antibody.
anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) antibody (2) anti-SH3 Domain Containing Ring Finger 1 (SH3RF1) antibody (Image 2)
Background publications Kärkkäinen, Hiipakka, Wang et al.: "Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome." in: EMBO reports, Vol. 7, Issue 2, pp. 186-91, 2006 (PubMed).

Lyons, Thorburn, Ryan et al.: "Regulation of the Pro-apoptotic scaffolding protein POSH by Akt." in: The Journal of biological chemistry, Vol. 282, Issue 30, pp. 21987-97, 2007 (PubMed).

Votteler, Iavnilovitch, Fingrut et al.: "Exploring the functional interaction between POSH and ALIX and the relevance to HIV-1 release." in: BMC biochemistry, Vol. 10, pp. 12, 2009 (PubMed).

Lin, Yue, Pan et al.: "POSH stimulates the ubiquitination and the clathrin-independent endocytosis of ROMK1 channels." in: The Journal of biological chemistry, Vol. 284, Issue 43, pp. 29614-24, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Hosts (20), (4)
Reactivities (23), (17), (16), (13), (12), (12), (1)
Applications (14), (10), (9), (3), (3), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (1), (1)
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