RAD18 Homolog (S. Cerevisiae) (RAD18) antibody

Antigen: RAD18 Homolog (S. Cerevisiae) (RAD18)

Synonyms: RNF73, Rad18sc, 2810024C04Rik, MGC156682, MGC55577, zgc:55577, RAD18, MGC134015, DKFZp469H0810

Clonality: Monoclonal (3H7)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393742

Additional Information

Alternative name: RAD18

Gene ID: 56852

UniProt: NP_064550

Immunogen: RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3H7

Description: Other names: RNF73 RAD18 homolog (S. cerevisiae)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 56195 DA

Comments:

Background: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to RAD18 (AT3545a) on HeLa cell . [antibody concentration 25 ug/ml] Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi ( Cat # H00056852-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody (M01), clone 3H7 (Cat # AT3545a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-RAD18 Homolog (S. Cerevisiae) (RAD18) antibody (Image 2)

Publications

Product: Yoshimura, Seki, Kanamori et al.: "Physical and functional interaction between WRNIP1 and RAD18." in: Genes & genetic systems, Vol. 84, Issue 2, pp. 171-8, 2009 (PubMed).

Nakamura, Ishikawa, Koga et al.: "Mutation analysis of Rad18 in human cancer cell lines and non small cell lung cancer tissues." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 106, 2009 (PubMed).

Shen, Gammon, Wu et al.: "Multiple genetic variants in telomere pathway genes and breast cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 1, pp. 219-28, 2010 (PubMed).

Yang, Moldovan, DAndrea: "RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger." in: The Journal of biological chemistry, Vol. 285, Issue 25, pp. 19085-91, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).