RAD18 Homolog (S. Cerevisiae) (RAD18) antibody

Details for Product No. ABIN393742
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Antigen
Synonyms RNF73, Rad18sc, 2810024C04Rik, zgc:55577, RAD18, DDBDRAFT_0218120, DDBDRAFT_0304878, DDB_0218120, DDB_0304878, DKFZp469H0810
Reactivity
Human
(35), (19), (6), (1), (1), (1), (1)
Host
Mouse
(30), (7)
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
(36), (17), (4), (4), (3), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393742
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3H7
Isotype IgG2b kappa
Specificity RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RAD18
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 56195 DA
Gene ID 12406
UniProt NP_064550
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-RAD18 Homolog (S. Cerevisiae) (RAD18) antibody Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell (antibody concentration 25 µg/mL). Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi used as specificity and loading control.
anti-RAD18 Homolog (S. Cerevisiae) (RAD18) antibody (2) anti-RAD18 Homolog (S. Cerevisiae) (RAD18) antibody (Image 2)
Background publications Yoshimura, Seki, Kanamori et al.: "Physical and functional interaction between WRNIP1 and RAD18." in: Genes & genetic systems, Vol. 84, Issue 2, pp. 171-8, 2009 (PubMed).

Nakamura, Ishikawa, Koga et al.: "Mutation analysis of Rad18 in human cancer cell lines and non small cell lung cancer tissues." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 106, 2009 (PubMed).

Shen, Gammon, Wu et al.: "Multiple genetic variants in telomere pathway genes and breast cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 1, pp. 219-28, 2010 (PubMed).

Yang, Moldovan, DAndrea: "RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger." in: The Journal of biological chemistry, Vol. 285, Issue 25, pp. 19085-91, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Hosts (30), (7)
Reactivities (35), (19), (6), (1), (1), (1), (1)
Applications (36), (17), (4), (4), (3), (2), (2), (1)
Epitopes (10), (3), (3), (2), (1), (1), (1), (1)
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