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RAD18 Homolog (S. Cerevisiae) (RAD18) (AA 332-431) antibody

Details for Product No. ABIN393742, Supplier: Log in to see
Antigen
  • zgc:55577
  • RAD18
  • DDBDRAFT_0218120
  • DDBDRAFT_0304878
  • DDB_0218120
  • DDB_0304878
  • DKFZp469H0810
  • RNF73
  • 2810024C04Rik
  • Rad18sc
Epitope
AA 332-431
8
7
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
62
17
10
3
2
1
1
1
Host
Mouse
51
12
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
48
23
15
8
7
6
2
1
1
1
1
Supplier
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Immunogen RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3H7
Isotype IgG2b kappa
Specificity RAD18 (NP_064550, 332 a.a. ~ 431 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RAD18 (RAD18 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 56195 DA
Gene ID 12406
NCBI Accession NP_064550
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-RAD18 Homolog (S. Cerevisiae) (RAD18) (AA 332-431) antibody (ABIN393742) Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-RAD18 Homolog (S. Cerevisiae) (RAD18) (AA 332-431) antibody (ABIN393742) anti-RAD18 Homolog (S. Cerevisiae) (RAD18) (AA 332-431) antibody (Image 2)
Background publications Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Yang, Moldovan, DAndrea: "RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger." in: The Journal of biological chemistry, Vol. 285, Issue 25, pp. 19085-91, 2010 (PubMed).

Shen, Gammon, Wu et al.: "Multiple genetic variants in telomere pathway genes and breast cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 1, pp. 219-28, 2010 (PubMed).

Nakamura, Ishikawa, Koga et al.: "Mutation analysis of Rad18 in human cancer cell lines and non small cell lung cancer tissues." in: Journal of experimental & clinical cancer research : CR, Vol. 28, pp. 106, 2009 (PubMed).

Yoshimura, Seki, Kanamori et al.: "Physical and functional interaction between WRNIP1 and RAD18." in: Genes & genetic systems, Vol. 84, Issue 2, pp. 171-8, 2009 (PubMed).