E4F1 antibody (E4F Transcription Factor 1) (AA 685-785)

Details for Product anti-E4F1 Antibody No. ABIN393753, Supplier: Log in to see
Antigen
  • e4f
  • MGC147041
  • E4F
  • Dnase1l2
  • zgc:114190
  • E4F transcription factor 1
  • E4F1
  • e4f1
  • LOC100224725
  • E4f1
Epitope
AA 685-785
7
4
3
3
3
1
1
Reactivity
Human
21
2
2
1
1
1
1
1
Host
Mouse
13
8
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
20
9
5
4
2
2
1
1
Options
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Immunogen E4F1 (NP_004415, 685 a.a. ~ 785 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4B10
Isotype IgG2a kappa
Specificity E4F1 (NP_004415, 685 a.a. ~ 785 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name E4F1 (E4F1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 83477 DA
Gene ID 12406
NCBI Accession NP_004415
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-E4F1 antibody (E4F Transcription Factor 1) (AA 685-785) (ABIN393753) Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monocl...
Background publications Cvetanovic, Rooney, Garcia, Toporovskaya, Zoghbi, Opal: "The role of LANP and ataxin 1 in E4F-mediated transcriptional repression." in: EMBO reports, Vol. 8, Issue 7, pp. 671-7, 2007 (PubMed).

Le Cam, Linares, Paul, Julien, Lacroix, Hatchi, Triboulet, Bossis, Shmueli, Rodriguez, Coux, Sardet: "E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation." in: Cell, Vol. 127, Issue 4, pp. 775-88, 2006 (PubMed).

Paul, Lacroix, Iankova, Julien, Schäfer, Labalette, Wei, Le Cam, Le Cam, Sardet: "The LIM-only protein FHL2 is a negative regulator of E4F1." in: Oncogene, Vol. 25, Issue 40, pp. 5475-84, 2006 (PubMed).

Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Nakamura, Igarashi, Suzuki, Kanno, Inoue, Tazawa, Saruta, Ando, Moriyama, Furukawa, Ono, Moriya, Ito, Saito, Ishibashi, Takahashi, Yamada, Sasano: "E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2019-31, 2004 (PubMed).