E4F Transcription Factor 1 (E4F1) antibody

Antigen: E4F Transcription Factor 1 (E4F1)

Synonyms: E4F, MGC99614, e4f, MGC147041, MGC114190, zgc:114190, Dnase1l2

Clonality: Monoclonal (4B10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393753

Additional Information

Alternative name: E4F1

Gene ID: 1877

UniProt: NP_004415

Immunogen: E4F1 (NP_004415, 685 a.a. ~ 785 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4B10

Description: Other names: E4F, MGC99614 E4F transcription factor 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: E4F1 (NP_004415, 685 a.a. ~ 785 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 83477 DA

Comments:

Background: The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysate(83.5 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Nakamura, Igarashi, Suzuki et al.: "E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2019-31, 2004 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Paul, Lacroix, Iankova et al.: "The LIM-only protein FHL2 is a negative regulator of E4F1." in: Oncogene, Vol. 25, Issue 40, pp. 5475-84, 2006 (PubMed).

Le Cam, Linares, Paul et al.: "E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation." in: Cell, Vol. 127, Issue 4, pp. 775-88, 2006 (PubMed).

Cvetanovic, Rooney, Garcia et al.: "The role of LANP and ataxin 1 in E4F-mediated transcriptional repression." in: EMBO reports, Vol. 8, Issue 7, pp. 671-7, 2007 (PubMed).