E4F Transcription Factor 1 (E4F1) antibody

Details for Product No. ABIN393753
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms E4F, Dnase1l2, e4f, MGC147041, zgc:114190
Reactivity
Human
(23), (12), (12), (12), (12), (12)
Host
Mouse
(19), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(10), (10), (8), (4), (3), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393753
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen E4F1 (NP_004415, 685 a.a. ~ 785 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4B10
Isotype IgG2a kappa
Specificity E4F1 (NP_004415, 685 a.a. ~ 785 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name E4F1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 83477 DA
Gene ID 12406
UniProt NP_004415
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-E4F Transcription Factor 1 (E4F1) antibody Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysate(83.5 KDa). Lane 2: Non-transfected lysate.
Background publications Nakamura, Igarashi, Suzuki et al.: "E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis." in: The American journal of pathology, Vol. 165, Issue 6, pp. 2019-31, 2004 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Paul, Lacroix, Iankova et al.: "The LIM-only protein FHL2 is a negative regulator of E4F1." in: Oncogene, Vol. 25, Issue 40, pp. 5475-84, 2006 (PubMed).

Le Cam, Linares, Paul et al.: "E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation." in: Cell, Vol. 127, Issue 4, pp. 775-88, 2006 (PubMed).

Cvetanovic, Rooney, Garcia et al.: "The role of LANP and ataxin 1 in E4F-mediated transcriptional repression." in: EMBO reports, Vol. 8, Issue 7, pp. 671-7, 2007 (PubMed).

Hosts (19), (4)
Reactivities (23), (12), (12), (12), (12), (12)
Applications (10), (10), (8), (4), (3), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top