V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTry207) antibody

Antigen: V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL)

Synonyms: Crkol, AA589403, AI325100, 1110025F07Rik, CRKL, MGC63469, zgc:63469, wu:fa55g05, wu:fj60e01

Epitope: pTry207

Clonality: Monoclonal (4B5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393757

Additional Information

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Alternative name: CRKL

Gene ID: 1399

Swiss-Prot: NP_005198

Immunogen: CRKL (NP_005198, 204 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: 4B5

Description: Other names: v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Molecular Weight: 33777 DA

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi ( Cat # H00001399-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody (M03), clone 4B5 (Cat # AT1624a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody (M03), clone 4B5. Lane 1: CRKL transfected lysate(33.8 KDa). Lane 2: Non-transfected lysate.

anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (pTry207) antibody (Image 2)