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Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody

Details for Product No. ABIN393759
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Antigen
Synonyms CHK-2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, HUCDS1, Rad53, Chk2, fa66f08, wu:fa66f08, zgc:55865
Epitope
AA 1-544
(36), (36), (28), (28), (16), (13), (13), (11), (9), (7), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(332), (146), (145), (55), (37), (36), (12), (7), (6), (1)
Host
Mouse
(273), (64), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(257), (109), (89), (60), (58), (41), (31), (28), (9), (4), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393759
450.00 $
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Immunogen CHEK2 (AAH04207, 1 a.a. ~ 544 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4B7
Isotype IgG2a kappa
Specificity CHEK2 (AAH04207, 1 a.a. ~ 544 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CHEK2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 60915 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell (antibody concentration 10 µg/mL). CHEK2 monoclonal antibody (M01), clone 4B7. Western Blot analysis of CHEK2 expression in HeLa.
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (2) anti-Checkpoint Kinase 2 (CHEK2) (AA 1-544) antibody (Image 2)
Background publications Evans, Ahmed, Bayliss et al.: "BRCA1, BRCA2 and CHEK2 c.1100 delC mutations in patients with double primaries of the breasts and/or ovaries." in: Journal of medical genetics, Vol. 47, Issue 8, pp. 561-6, 2010 (PubMed).

Monsees, Kraft, Chanock et al.: "Comprehensive screen of genetic variation in DNA repair pathway genes and postmenopausal breast cancer risk." in: Breast cancer research and treatment, Vol. 125, Issue 1, pp. 207-14, 2010 (PubMed).

Ho-Pun-Cheung, Assenat, Bascoul-Mollevi et al.: "A large-scale candidate gene approach identifies SNPs in SOD2 and IL13 as predictive markers of response to preoperative chemoradiation in rectal cancer." in: The pharmacogenomics journal, Vol. 11, Issue 6, pp. 437-43, 2011 (PubMed).

Konstantinova, Kadiyska, Sokolova et al.: "CHEK2 I157T and colorectal cancer in Bulgaria." in: Journal of B.U.ON. : official journal of the Balkan Union of Oncology, Vol. 15, Issue 2, pp. 314-7, 2010 (PubMed).

Guénard, Pedneault, Ouellette et al.: "Evaluation of the contribution of the three breast cancer susceptibility genes CHEK2, STK11, and PALB2 in non-BRCA1/2 French Canadian families with high risk of breast cancer." in: Genetic testing and molecular biomarkers, Vol. 14, Issue 4, pp. 515-26, 2010 (PubMed).

Validation Images
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