Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1) antibody

Antigen: Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1)

Synonyms: UCP1, ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1, ATPUMP1, ATUCP1, PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1, PUMP1, UCP, UNCOUPLING PROTEIN 1, SLC25A7, Ucp, Slc25a7, AI385626, Ucpa, Uncp, MGC108736, ucp, slc25a7

Clonality: Monoclonal (4B7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393760

Additional Information

Alternative name: UCP1

Gene ID: 7350

UniProt: NP_068605

Immunogen: UCP1 (NP_068605, 232 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4B7

Description: Other names: SLC25A7, UCP uncoupling protein 1 (mitochondrial, proton carrier)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: UCP1 (NP_068605, 232 a.a. ~ 268 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 33005 DA

Comments:

Background: Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Synonyms: UCP1, ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1, ATPUMP1, ATUCP1, PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1, PUMP1, UCP, UNCOUPLING PROTEIN 1, SLC25A7, Ucp, Slc25a7, AI385626, Ucpa, Uncp, MGC108736, ucp, slc25a7

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

UCP1 monoclonal antibody (M03), clone 4B7. Western Blot analysis of UCP1 expression in NIH/3T3 ( Cat # L018V1 ).

Publications

Product: Pinheiro, Bulik, Thornton et al.: "Association study of 182 candidate genes in anorexia nervosa." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 153B, Issue 5, pp. 1070-80, 2010 (PubMed).

Wang, Li, Glessner et al.: "Large copy-number variations are enriched in cases with moderate to extreme obesity." in: Diabetes, Vol. 59, Issue 10, pp. 2690-4, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Hancock, Clark, Qian et al.: "Population genetic analysis of the uncoupling proteins supports a role for UCP3 in human cold resistance." in: Molecular biology and evolution, Vol. 28, Issue 1, pp. 601-14, 2010 (PubMed).

Kim, Lee: "The effects of uncoupling protein 1 and beta3-adrenergic receptor gene polymorphisms on weight loss and lipid profiles in obese women." in: International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition, Vol. 80, Issue 2, pp. 87-96, 2010 (PubMed).