|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Coilin (COIL) antibody
|Synonyms||COIL, MGC134097, p80c, coil, CLN80, wu:fb08h11, p80-coilin, Cln80, C79982, MGC37472, DmelCG8710, CG8710, sph1, LOC100218802, F3F19.5, F3F19_5|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Quantity||200 uL mg|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||COIL (NP_004636, 477 a.a. ~ 576 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: CLN80, p80coilin coilin|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||COIL (NP_004636, 477 a.a. ~ 576 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||62608 DA|
Background: The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
|COIL monoclonal antibody (M05A), clone 4B8 Western Blot analysis of COIL expression in NIH/3T3 ( Cat # L018V1 ).|
Hong, Lee, Cho et al.: "UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1." in: Biochemical and biophysical research communications, Vol. 371, Issue 2, pp. 256-60, 2008 (PubMed).
Bongiorno-Borbone, De Cola, Vernole et al.: "FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 15, pp. 2357-67, 2008 (PubMed).
Hearst, Gilder, Negi et al.: "Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines." in: Journal of cell science, Vol. 122, Issue Pt 11, pp. 1872-81, 2009 (PubMed).
Toyota, Davis, Cosman et al.: "Coilin phosphorylation mediates interaction with SMN and SmB'." in: Chromosoma, Vol. 119, Issue 2, pp. 205-15, 2010 (PubMed).
Zhao, Li, Bradfield et al.: "The role of height-associated loci identified in genome wide association studies in the determination of pediatric stature." in: BMC medical genetics, Vol. 11, pp. 96, 2010 (PubMed).
|Hosts||Rabbit (11), Mouse (3)|
|Reactivities||Human (12), Mouse (Murine) (5), Rat (Rattus) (5), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Chicken (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (13), ELISA (10), Immunofluorescence (IF) (5), Immunohistochemistry (IHC) (5), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Immunoprecipitation (IP) (1)|
|Epitopes||C-Term (2), Center (1)|