Coilin (COIL) antibody

Antigen: Coilin (COIL)

Synonyms: COIL, MGC134097, p80c, coil, CLN80, wu:fb08h11, p80-coilin, Cln80, C79982, MGC37472, DmelCG8710, CG8710, sph1, LOC100218802, F3F19.5, F3F19_5

Clonality: Monoclonal (4B8)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393763

Additional Information

Alternative name: COIL

Gene ID: 8161

UniProt: NP_004636

Immunogen: COIL (NP_004636, 477 a.a. ~ 576 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgM kappa

Clone: 4B8

Description: Other names: CLN80, p80coilin coilin

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: COIL (NP_004636, 477 a.a. ~ 576 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 62608 DA

Comments:

Background: The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

COIL monoclonal antibody (M05A), clone 4B8 Western Blot analysis of COIL expression in NIH/3T3 ( Cat # L018V1 ).

Publications

Product: Hong, Lee, Cho et al.: "UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1." in: Biochemical and biophysical research communications, Vol. 371, Issue 2, pp. 256-60, 2008 (PubMed).

Bongiorno-Borbone, De Cola, Vernole et al.: "FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 15, pp. 2357-67, 2008 (PubMed).

Hearst, Gilder, Negi et al.: "Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines." in: Journal of cell science, Vol. 122, Issue Pt 11, pp. 1872-81, 2009 (PubMed).

Toyota, Davis, Cosman et al.: "Coilin phosphorylation mediates interaction with SMN and SmB'." in: Chromosoma, Vol. 119, Issue 2, pp. 205-15, 2010 (PubMed).

Zhao, Li, Bradfield et al.: "The role of height-associated loci identified in genome wide association studies in the determination of pediatric stature." in: BMC medical genetics, Vol. 11, pp. 96, 2010 (PubMed).