A Kinase (PRKA) Anchor Protein 4 (AKAP4) antibody

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Synonyms AKAP 82, AKAP-4, AKAP82, CT99, FSC1, HI, PRKA4, hAKAP82, p82, Fsc1, AKAP4
(6), (5)
Clonality (Clone)
Monoclonal ()
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(10), (6), (2), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393766
Quantity 0.1 mg
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Immunogen AKAP4 (NP_003877, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C10
Isotype IgG2a kappa
Specificity AKAP4 (NP_003877, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKAP4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 94477 DA
Gene ID 12406
UniProt NP_003877
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-A Kinase (PRKA) Anchor Protein 4 (AKAP4) antibody Immunoperoxidase of monoclonal antibody to AKAP4 on formalin-fixed paraffin-embedded human testis (antibody concentration 1 µg/mL). Detection limit for recombinant GST tagged AKAP4 is approximately 0.1 ng/mL as a capture antibody.
anti-A Kinase (PRKA) Anchor Protein 4 (AKAP4) antibody (2) anti-A Kinase (PRKA) Anchor Protein 4 (AKAP4) antibody (Image 2)
Background publications Brown, Miki, Harper et al.: "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum." in: Biology of reproduction, Vol. 68, Issue 6, pp. 2241-8, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ross, Grafham, Coffey et al.: "The DNA sequence of the human X chromosome...." in: Nature, Vol. 434, Issue 7031, pp. 325-37, 2005 (PubMed).

Lefièvre, Chen, Conner et al.: "Human spermatozoa contain multiple targets for protein S-nitrosylation: an alternative mechanism of the modulation of sperm function by nitric oxide?" in: Proteomics, Vol. 7, Issue 17, pp. 3066-84, 2007 (PubMed).

Moretti, Scapigliati, Pascarelli et al.: "Localization of AKAP4 and tubulin proteins in sperm with reduced motility." in: Asian journal of andrology, Vol. 9, Issue 5, pp. 641-9, 2007 (PubMed).

Hosts (6), (5)
Reactivities (11)
Applications (10), (6), (2), (2), (1)
Epitopes (3), (2), (2), (2), (1)
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