UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 6 (GalNAc-T6) (GALNT6) antibody

Antigen: UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 6 (GalNAc-T6) (GALNT6)

Clonality: Monoclonal (4C10)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393767

Additional Information

Alternative name: GALNT6

Gene ID: 11226

UniProt: NP_009141

Immunogen: GALNT6 (NP_009141, 523 a.a. ~ 623 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 4C10

Description: Other names: GALNACT6, GalNAcT6 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: GALNT6 (NP_009141, 523 a.a. ~ 623 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 71159 DA

Comments:

Background: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of GALNT6 over-expressed 293 cell line, cotransfected with GALNT6 Validated Chimera RNAi ( Cat # H00011226-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GALNT6 monoclonal antibody (M01), clone 4C10 (Cat # AT2147a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of GALNT6 expression in transfected 293T cell line by GALNT6 monoclonal antibody (M01), clone 4C10. Lane 1: GALNT6 transfected lysate(71 KDa). Lane 2: Non-transfected lysate.

anti-UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 6 (GalNAc-T6) (GALNT6) antibody (Image 2)

Publications

Product: Otsuki, Ota, Nishikawa et al.: "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 12, Issue 2, pp. 117-26, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Gomes, Marcos, Berois et al.: "Expression of UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6 in gastric mucosa, intestinal metaplasia, and gastric carcinoma." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 1, pp. 79-86, 2008 (PubMed).

Patani, Jiang, Mokbel: "Prognostic utility of glycosyltransferase expression in breast cancer." in: Cancer genomics & proteomics, Vol. 5, Issue 6, pp. 333-40, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).