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Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 63-153) antibody

Details for Product No. ABIN393775, Supplier: Log in to see
Antigen
  • fc79h10
  • wu:fc79h10
  • zgc:100921
  • e2-17kda
  • hhr6b
  • hr6b
  • rad6b
  • ubc2
  • E2-17kDa
  • HHR6B
  • HR6B
  • RAD6B
  • UBC2
  • 2610301N02Rik
  • E2-14k
  • Rad6b
  • mHR6B
Epitope
AA 63-153
18
5
3
3
2
1
1
1
1
1
Reactivity
Human
45
9
5
1
Host
Mouse
34
11
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
39
23
20
3
3
1
1
1
Supplier
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Immunogen UBE2B (NP_003328, 63 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C3
Isotype IgG2a kappa
Specificity UBE2B (NP_003328, 63 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2B (UBE2B Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17312 DA
Gene ID 12406
NCBI Accession NP_003328
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 63-153) antibody (ABIN393775) Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B mono...
Background publications Aston, Krausz, Laface et al.: "Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent." in: Human reproduction (Oxford, England), Vol. 25, Issue 6, pp. 1383-97, 2010 (PubMed).

Ryu, Choi, Ko et al.: "Direct characterization of E2-dependent target specificity and processivity using an artificial p27-linker-E2 ubiquitination system." in: BMB reports, Vol. 41, Issue 12, pp. 852-7, 2009 (PubMed).

Suryavathi, Khattri, Gopal et al.: "Novel variants in UBE2B gene and idiopathic male infertility." in: Journal of andrology, Vol. 29, Issue 5, pp. 564-71, 2008 (PubMed).

Huang, Emery, Christensen et al.: "Novel UBE2B-associated polymorphisms in an azoospermic/oligozoospermic population." in: Asian journal of andrology, Vol. 10, Issue 3, pp. 461-6, 2008 (PubMed).

Tsuji, Watanabe, Araki et al.: "Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, 2008 (PubMed).