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Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 63-153) antibody

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Synonyms fc79h10, wu:fc79h10, zgc:100921, e2-17kda, hhr6b, hr6b, rad6b, ubc2, E2-17kDa, HHR6B, HR6B, RAD6B, UBC2, 2610301N02Rik, E2-14k, Rad6b, mHR6B
AA 63-153
(18), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
(47), (9), (5), (1)
(36), (11)
Clonality (Clone)
Monoclonal ()
(2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(41), (23), (21), (4), (3), (2), (1), (1)
Pubmed 5 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 8 to 11 Business Days
Immunogen UBE2B (NP_003328, 63 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C3
Isotype IgG2a kappa
Specificity UBE2B (NP_003328, 63 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2B (UBE2B Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17312 DA
Gene ID 12406
NCBI Accession NP_003328
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2B (UBE2B) (AA 63-153) antibody (ABIN393775) Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B mono...
Background publications Aston, Krausz, Laface et al.: "Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent." in: Human reproduction (Oxford, England), Vol. 25, Issue 6, pp. 1383-97, 2010 (PubMed).

Ryu, Choi, Ko et al.: "Direct characterization of E2-dependent target specificity and processivity using an artificial p27-linker-E2 ubiquitination system." in: BMB reports, Vol. 41, Issue 12, pp. 852-7, 2009 (PubMed).

Suryavathi, Khattri, Gopal et al.: "Novel variants in UBE2B gene and idiopathic male infertility." in: Journal of andrology, Vol. 29, Issue 5, pp. 564-71, 2008 (PubMed).

Huang, Emery, Christensen et al.: "Novel UBE2B-associated polymorphisms in an azoospermic/oligozoospermic population." in: Asian journal of andrology, Vol. 10, Issue 3, pp. 461-6, 2008 (PubMed).

Tsuji, Watanabe, Araki et al.: "Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, 2008 (PubMed).

Catalog No. ABIN393775
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