Ubiquitin-Conjugating Enzyme E2B (UBE2B) antibody

Details for Product No. ABIN393775
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms HR6B, UBC2, HHR6B, RAD6B, E2-17kDa, Rad6b, mHR6B, E2-14k, 2610301N02Rik, hhr6b, hr6b, rad6b, ubc2, fc79h10, wu:fc79h10, zgc:100921, e2-17kda
Reactivity
Human
(30), (16), (15), (12), (1), (1), (1), (1)
Host
Mouse
(23), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(19), (13), (10), (6), (3), (2), (1)
Pubmed 5 references available
Catalog no. ABIN393775
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen UBE2B (NP_003328, 63 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4C3
Isotype IgG2a kappa
Specificity UBE2B (NP_003328, 63 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name UBE2B
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 17312 DA
Gene ID 12406
UniProt NP_003328
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2B (UBE2B) antibody Western Blot analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M06), clone 4C3. Lane 1: UBE2B transfected lysate(17.3 KDa). Lane 2: Non-transfected lysate.
Background publications Tsuji, Watanabe, Araki et al.: "Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, 2008 (PubMed).

Huang, Emery, Christensen et al.: "Novel UBE2B-associated polymorphisms in an azoospermic/oligozoospermic population." in: Asian journal of andrology, Vol. 10, Issue 3, pp. 461-6, 2008 (PubMed).

Suryavathi, Khattri, Gopal et al.: "Novel variants in UBE2B gene and idiopathic male infertility." in: Journal of andrology, Vol. 29, Issue 5, pp. 564-71, 2008 (PubMed).

Ryu, Choi, Ko et al.: "Direct characterization of E2-dependent target specificity and processivity using an artificial p27-linker-E2 ubiquitination system." in: BMB reports, Vol. 41, Issue 12, pp. 852-7, 2009 (PubMed).

Aston, Krausz, Laface et al.: "Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent." in: Human reproduction (Oxford, England), Vol. 25, Issue 6, pp. 1383-97, 2010 (PubMed).

Hosts (23), (7)
Reactivities (30), (16), (15), (12), (1), (1), (1), (1)
Applications (19), (13), (10), (6), (3), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top