Dual Specificity Phosphatase 5 (DUSP5) antibody

Antigen: Dual Specificity Phosphatase 5 (DUSP5)

Synonyms: DUSP, HVH3, DUSP5, wu:fc03b11, wu:fc86e02, Cpg21, Gm337, MGC84792, dusp, hvh3, MGC108270

Clonality: Monoclonal (4C8)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393778

Additional Information

Alternative name: DUSP5

Gene ID: 1847

UniProt: NP_004410

Immunogen: DUSP5 (NP_004410, 286 a.a. ~ 385 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 4C8

Description: Other names: DUSP, HVH3dual specificity phosphatase 5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DUSP5 (NP_004410, 286 a.a. ~ 385 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 42107 DA

Comments:

Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of to DUSP5 ( Cat# AT1828a ) on HeLa cell . DUSP5 monoclonal antibody (M03), clone 4C8 Western Blot analysis of DUSP5 expression in K-562 ( Cat # L009V1 ).

anti-Dual Specificity Phosphatase 5 (DUSP5) antibody (Image 2)

Publications

Product: Pramanik, Chun, Garnaas et al.: "Dusp-5 and Snrk-1 coordinately function during vascular development and disease." in: Blood, Vol. 113, Issue 5, pp. 1184-91, 2009 (PubMed).

Grasset, Gobert-Gosse, Mouchiroud et al.: "Macrophage differentiation of myeloid progenitor cells in response to M-CSF is regulated by the dual-specificity phosphatase DUSP5." in: Journal of leukocyte biology, Vol. 87, Issue 1, pp. 127-35, 2010 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).