Metastasis Associated 1 (MTA1) antibody

Antigen: Metastasis Associated 1 (MTA1)

Synonyms: MGC118456, MGC83916

Clonality: Monoclonal (4D11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393781

Additional Information

Alternative name: MTA1

Gene ID: 9112

UniProt: NP_004680

Immunogen: MTA1 (NP_004680, 601 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa

Clone: 4D11

Description: Other names: metastasis associated 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: MTA1 (NP_004680, 601 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 80788 DA

Comments:

Background: This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to MTA1 (AT2923a) on HeLa cell . [antibody concentration 10 ug/ml] MTA1 monoclonal antibody (M02), clone 4D11. Western Blot analysis of MTA1 expression in NIH/3T3 ( Cat # L018V1 ).

anti-Metastasis Associated 1 (MTA1) antibody (Image 2)

Publications

Product: Bui-Nguyen, Pakala, Sirigiri et al.: "Stimulation of inducible nitric oxide by hepatitis B virus transactivator protein HBx requires MTA1 coregulator." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 6980-6, 2010 (PubMed).

Li, Pakala, Reddy et al.: "Revelation of p53-independent function of MTA1 in DNA damage response via modulation of the p21 WAF1-proliferating cell nuclear antigen pathway." in: The Journal of biological chemistry, Vol. 285, Issue 13, pp. 10044-52, 2010 (PubMed).

Chokas, Trivedi, Lu et al.: "Foxp1/2/4-NuRD interactions regulate gene expression and epithelial injury response in the lung via regulation of interleukin-6." in: The Journal of biological chemistry, Vol. 285, Issue 17, pp. 13304-13, 2010 (PubMed).

Li, Ohshiro, Khan et al.: "Requirement of MTA1 in ATR-mediated DNA damage checkpoint function." in: The Journal of biological chemistry, Vol. 285, Issue 26, pp. 19802-12, 2010 (PubMed).

Van Rechem, Boulay, Pinte et al.: "Differential regulation of HIC1 target genes by CtBP and NuRD, via an acetylation/SUMOylation switch, in quiescent versus proliferating cells." in: Molecular and cellular biology, Vol. 30, Issue 16, pp. 4045-59, 2010 (PubMed).