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Sin3A-Associated Protein, 30kDa (SAP30) (AA 131-220) antibody

Details for Product No. ABIN393782
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Antigen
Synonyms RGD1309585, 30kDa
Epitope
AA 131-220
(8), (7), (6), (3), (3), (2), (2), (1), (1), (1)
Reactivity
Human
(28), (2), (2), (2), (2), (1), (1)
Host
Mouse
(15), (13)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(28), (17), (6), (4), (3), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393782
450.00 $
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Immunogen SAP30 (NP_003855, 131 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D2
Isotype IgG2b kappa
Specificity SAP30 (NP_003855, 131 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAP30
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23306 DA
Gene ID 12406
UniProt NP_003855
Research Area Signaling, Protein Modifications, Chromatin, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Sin3A-Associated Protein, 30kDa (SAP30) (AA 131-220) antibody Immunoperoxidase of monoclonal antibody to SAP30 on formalin-fixed paraffin-embedded human esophagus (antibody concentration 1.5 µg/mL). Detection limit for recombinant GST tagged SAP30 is approximately 1 ng/mL as a capture antibody.
anti-Sin3A-Associated Protein, 30kDa (SAP30) (AA 131-220) antibody (2) anti-Sin3A-Associated Protein, 30kDa (SAP30) (AA 131-220) antibody (Image 2)
Background publications Doyon, Cayrou, Ullah et al.: "ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation." in: Molecular cell, Vol. 21, Issue 1, pp. 51-64, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Szafranski, Schindler, Taudien et al.: "Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns." in: Genome biology, Vol. 8, Issue 8, pp. R154, 2008 (PubMed).

Sichtig, Körfer, Steger: "Papillomavirus binding factor binds to SAP30 and represses transcription via recruitment of the HDAC1 co-repressor complex." in: Archives of biochemistry and biophysics, Vol. 467, Issue 1, pp. 67-75, 2007 (PubMed).

Viiri, Jänis, Siggers et al.: "DNA-binding and -bending activities of SAP30L and SAP30 are mediated by a zinc-dependent module and monophosphoinositides." in: Molecular and cellular biology, Vol. 29, Issue 2, pp. 342-56, 2008 (PubMed).

Validation Images
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