Sin3A-Associated Protein, 30kDa (SAP30) antibody

Antigen: Sin3A-Associated Protein, 30kDa (SAP30)

Clonality: Monoclonal (4D2)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393782

Additional Information

Alternative name: SAP30

Gene ID: 8819

UniProt: NP_003855

Immunogen: SAP30 (NP_003855, 131 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 4D2

Description: Other names: Sin3A-associated protein, 30kDa

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SAP30 (NP_003855, 131 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 23306 DA

Comments:

Background: Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to SAP30 ( Cat# AT3771a ) on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1.5 ug/ml] Detection limit for recombinant GST tagged SAP30 is approximately 1ng/ml as a capture antibody.

anti-Sin3A-Associated Protein, 30kDa (SAP30) antibody (Image 2)

Publications

Product: Doyon, Cayrou, Ullah et al.: "ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation." in: Molecular cell, Vol. 21, Issue 1, pp. 51-64, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Szafranski, Schindler, Taudien et al.: "Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns." in: Genome biology, Vol. 8, Issue 8, pp. R154, 2008 (PubMed).

Sichtig, Körfer, Steger: "Papillomavirus binding factor binds to SAP30 and represses transcription via recruitment of the HDAC1 co-repressor complex." in: Archives of biochemistry and biophysics, Vol. 467, Issue 1, pp. 67-75, 2007 (PubMed).

Viiri, Jänis, Siggers et al.: "DNA-binding and -bending activities of SAP30L and SAP30 are mediated by a zinc-dependent module and monophosphoinositides." in: Molecular and cellular biology, Vol. 29, Issue 2, pp. 342-56, 2008 (PubMed).