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Metastasis Associated 1 (MTA1) antibody
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||MTA1 (NP_004680, 601 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: metastasis associated 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||MTA1 (NP_004680, 601 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||80788 DA|
Background: This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Bui-Nguyen, Pakala, Sirigiri et al.: "Stimulation of inducible nitric oxide by hepatitis B virus transactivator protein HBx requires MTA1 coregulator." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 6980-6, 2010 (PubMed).
Li, Pakala, Reddy et al.: "Revelation of p53-independent function of MTA1 in DNA damage response via modulation of the p21 WAF1-proliferating cell nuclear antigen pathway." in: The Journal of biological chemistry, Vol. 285, Issue 13, pp. 10044-52, 2010 (PubMed).
Chokas, Trivedi, Lu et al.: "Foxp1/2/4-NuRD interactions regulate gene expression and epithelial injury response in the lung via regulation of interleukin-6." in: The Journal of biological chemistry, Vol. 285, Issue 17, pp. 13304-13, 2010 (PubMed).
Li, Ohshiro, Khan et al.: "Requirement of MTA1 in ATR-mediated DNA damage checkpoint function." in: The Journal of biological chemistry, Vol. 285, Issue 26, pp. 19802-12, 2010 (PubMed).
Van Rechem, Boulay, Pinte et al.: "Differential regulation of HIC1 target genes by CtBP and NuRD, via an acetylation/SUMOylation switch, in quiescent versus proliferating cells." in: Molecular and cellular biology, Vol. 30, Issue 16, pp. 4045-59, 2010 (PubMed).
|Hosts||Rabbit (30), Goat (6), Mouse (4)|
|Reactivities||Human (38), Mouse (Murine) (23), Rat (Rattus) (23), Chicken (2), Dog (Canine) (2), Cow (Bovine) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (23), Immunofluorescence (IF) (16), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Internal Region (3), C-Term (2), AA 400-450 (1), AA 601-701 (1)|