Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-551) antibody

Details for Product No. ABIN393792
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms Prkaa1, ampk, ampka1, PRKAA1, AMPK, AMPKa1, AMPKalpha1, AI194361, AI450832, AL024255, C130083N04Rik, cb116, im:7154392, sb:cb130, wu:fa94c10, AMPK1
Epitope
AA 451-551
(23), (19), (16), (14), (14), (12), (11), (10), (8), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(200), (119), (112), (30), (28), (28), (28), (4), (3), (1), (1), (1)
Host
Mouse
(188), (30), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application
Immunofluorescence (IF), Western Blotting (WB)
(162), (78), (61), (41), (41), (23), (19), (8), (7), (6), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Available
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN393792
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen PRKAA1 (AAH12622, 451 a.a. ~ 551 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4D8
Isotype IgG2a kappa
Specificity PRKAA1 (AAH12622, 451 a.a. ~ 551 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name PRKAA1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 62808 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-551) antibody Immunofluorescence of monoclonal antibody to PRKAA1 on HeLa cell (antibody concentration 10 µg/mL). PRKAA1 monoclonal antibody (M03), clone 4D8 Western Blot analysis of PRKAA1 expression in K-562.
anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-551) antibody (2) anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) (AA 451-551) antibody (Image 2)
Background publications Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Slattery, Herrick, Lundgreen et al.: "Genetic variation in a metabolic signaling pathway and colon and rectal cancer risk: mTOR, PTEN, STK11, RPKAA1, PRKAG2, TSC1, TSC2, PI3K and Akt1." in: Carcinogenesis, Vol. 31, Issue 9, pp. 1604-11, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jablonski, McAteer, de Bakker et al.: "Common variants in 40 genes assessed for diabetes incidence and response to metformin and lifestyle intervention in the diabetes prevention program." in: Diabetes, Vol. 59, Issue 10, pp. 2672-81, 2010 (PubMed).

Jassim, Fernø, Theisen et al.: "Association study of energy homeostasis genes and antipsychotic-induced weight gain in patients with schizophrenia." in: Pharmacopsychiatry, Vol. 44, Issue 1, pp. 15-20, 2011 (PubMed).

Validation Images
Did you look for something else?
back to top