Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibody

Antigen: Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1)

Synonyms: PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Clonality: Monoclonal (4D8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393792

Additional Information

Alternative name: PRKAA1

Gene ID: 5562

Immunogen: PRKAA1 (AAH12622, 451 a.a. ~ 551 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4D8

Description: Other names: 1, AMPK, AMPKa1, MGC33776, MGC57364, alpha protein kinase, AMP-activated, alpha 1 catalytic subunit

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: PRKAA1 (AAH12622, 451 a.a. ~ 551 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 62808 DA

Comments:

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Synonyms: PRKAA1, AMPK, AMPK1, AMPKa1, MGC33776, MGC57364, 5-AMP-activatedproteinkinasealpha-1catalyticsubunit, 5'-AMP-activatedproteinkinasealpha-1catalyticsubunit, AI194361, AI450832, AL024255, AMPKalpha1, C130083N04Rik, Prkaa1, MGC165837, cb116, sb:cb130, im:7154392, wu:fa94c10, DKFZp469M1511

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to PRKAA1 (AT3426a) on HeLa cell . [antibody concentration 10 ug/ml] PRKAA1 monoclonal antibody (M03), clone 4D8 Western Blot analysis of PRKAA1 expression in K-562 ( Cat # L009V1 ).

anti-Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1) antibody (Image 2)

Publications

Product: Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Slattery, Herrick, Lundgreen et al.: "Genetic variation in a metabolic signaling pathway and colon and rectal cancer risk: mTOR, PTEN, STK11, RPKAA1, PRKAG2, TSC1, TSC2, PI3K and Akt1." in: Carcinogenesis, Vol. 31, Issue 9, pp. 1604-11, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jablonski, McAteer, de Bakker et al.: "Common variants in 40 genes assessed for diabetes incidence and response to metformin and lifestyle intervention in the diabetes prevention program." in: Diabetes, Vol. 59, Issue 10, pp. 2672-81, 2010 (PubMed).

Jassim, Fernø, Theisen et al.: "Association study of energy homeostasis genes and antipsychotic-induced weight gain in patients with schizophrenia." in: Pharmacopsychiatry, Vol. 44, Issue 1, pp. 15-20, 2011 (PubMed).