Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) (AA 1201-1300) antibody

Details for Product No. ABIN393795
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms MAPKKK4, MEKK4, MTK1, PRO0412, D17Rp17, D17Rp17e, MEKK 4, Mek4b, Mekk4, RP17, Rp17a, Tas, mKIAA0213
Epitope
AA 1201-1300
(14), (8), (6), (2), (1), (1), (1)
Reactivity
Human
(53), (27), (14), (12), (12), (12), (12)
Host
Mouse
(36), (17)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(32), (31), (22), (11), (10), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 8 to 11 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN393795
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4F10
Isotype IgG1 kappa
Specificity MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP3K4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 181552 DA
Gene ID 12406
NCBI Accession NP_005913
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) (AA 1201-1300) antibody MAP3K4 monoclonal antibody (M08), clone 4F10 Western Blot analysis of MAP3K4 expression in NIH/3T3 on formalin-fixed paraffin-embedded human small Intestine (antibody concentration 1 µg/mL).
anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) (AA 1201-1300) antibody (2) anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) (AA 1201-1300) antibody (Image 2)
Background publications Derbyshire, Halfter, Heimark et al.: "Angiotensin II stimulated transcription of cyclooxygenase II is regulated by a novel kinase cascade involving Pyk2, MEKK4 and annexin II." in: Molecular and cellular biochemistry, Vol. 271, Issue 1-2, pp. 77-90, 2005 (PubMed).

Abell, Johnson: "MEKK4 is an effector of the embryonic TRAF4 for JNK activation." in: The Journal of biological chemistry, Vol. 280, Issue 43, pp. 35793-6, 2005 (PubMed).

Aissouni, Zapart, Iovanna et al.: "CIN85 regulates the ability of MEKK4 to activate the p38 MAP kinase pathway." in: Biochemical and biophysical research communications, Vol. 338, Issue 2, pp. 808-14, 2005 (PubMed).

Whitmarsh, Davis: "Role of mitogen-activated protein kinase kinase 4 in cancer." in: Oncogene, Vol. 26, Issue 22, pp. 3172-84, 2007 (PubMed).

Grucza, Johnson, Krueger et al.: "Incorporating age at onset of smoking into genetic models for nicotine dependence: evidence for interaction with multiple genes." in: Addiction biology, Vol. 15, Issue 3, pp. 346-57, 2010 (PubMed).

Validation Images
Did you look for something else?
back to top