Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody

Details for Product No. ABIN393796
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Antigen
Synonyms zgc:153726, CMM3, PSK-J3, Crk3, 8-6, CDK4, CDK4/6, CG5072, Cdk4/6, DmCdk4, Dmel\\CG5072, Pk53C, Pk?7, cdk, cdk4, cdk4/6, dCdk4, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, xcdk4
Epitope
AA 211-304
(42), (9), (8), (8), (7), (6), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(174), (65), (50), (20), (14), (5), (1)
Host
Mouse
(100), (83), (5), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (5), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(174), (62), (54), (38), (28), (17), (15), (10), (10), (9), (8), (5), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN393796
450.00 $
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Immunogen CDK4 (AAH03644, 211 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4F11
Isotype IgG1 kappa
Specificity CDK4 (AAH03644, 211 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CDK4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 33730 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell (antibody concentration 10 µg/mL). Western blot analysis of CDK4 over-expressed 293 cell line, cotransfected with CDK4 Validated Chimera RNAi used as specificity and loading control.
anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody (2) anti-Cyclin-Dependent Kinase 4 (CDK4) (AA 211-304) antibody (Image 2)
Background publications Michaud, Solomon, Oermann et al.: "Pharmacologic inhibition of cyclin-dependent kinases 4 and 6 arrests the growth of glioblastoma multiforme intracranial xenografts." in: Cancer research, Vol. 70, Issue 8, pp. 3228-38, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

Wiedemeyer, Dunn, Quayle et al.: "Pattern of retinoblastoma pathway inactivation dictates response to CDK4/6 inhibition in GBM." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11501-6, 2010 (PubMed).

Zeng, Shaikh, Harrison et al.: "The Ras oncogene signals centrosome amplification in mammary epithelial cells through cyclin D1/Cdk4 and Nek2." in: Oncogene, Vol. 29, Issue 36, pp. 5103-12, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Validation Images
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