serine Hydroxymethyltransferase 1 (Soluble) (SHMT1) antibody

Antigen: serine Hydroxymethyltransferase 1 (Soluble) (SHMT1)

Synonyms: Shmt, mshmt, C81125, mshmt1, mshmt2, AI324848, AI385541, mShmt, LRRGT00032, shmt1, MGC53442, MGC66171, zgc:66171, zgc:77524, SHMT1, MGC137164, MEL-32, Shmt1, DKFZp469J1932

Clonality: Monoclonal (4F9)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393802

Additional Information

Alternative name: SHMT1

Gene ID: 6470

UniProt: NP_004160

Immunogen: SHMT1 (NP_004160, 374 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 4F9

Description: Other names: CSHMT, MGC15229, MGC24556, SHMTserine hydroxymethyltransferase 1 (soluble)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SHMT1 (NP_004160, 374 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 53083 DA

Comments:

Background: This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

SHMT1 monoclonal antibody (M01), clone 4F9 Western Blot analysis of SHMT1 expression in HeLa ( Cat # L013V1 ). Immunoperoxidase of monoclonal antibody to SHMT1 ( Cat # AT3884a ) on formalin-fixed paraffin-embedded human colon tissue.

anti-serine Hydroxymethyltransferase 1 (Soluble) (SHMT1) antibody (Image 2)

Publications

Product: Vijayakrishnan, Houlston: "Candidate gene association studies and risk of childhood acute lymphoblastic leukemia: a systematic review and meta-analysis." in: Haematologica, Vol. 95, Issue 8, pp. 1405-14, 2010 (PubMed).

Summers, Mitchell, Stanislawska-Sachadyn et al.: "Genetic and lifestyle variables associated with homocysteine concentrations and the distribution of folate derivatives in healthy premenopausal women." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 88, Issue 8, pp. 679-88, 2010 (PubMed).

Levine, Figueiredo, Lee et al.: "A candidate gene study of folate-associated one carbon metabolism genes and colorectal cancer risk." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 7, pp. 1812-21, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Porter, Basu, Hubbard et al.: "Association of genetic variation in cystathionine-beta-synthase and arsenic metabolism." in: Environmental research, Vol. 110, Issue 6, pp. 580-7, 2010 (PubMed).