A Kinase (PRKA) Anchor Protein 4 (AKAP4) antibody

Antigen: A Kinase (PRKA) Anchor Protein 4 (AKAP4)

Synonyms: HI, p82, CT99, FSC1, AKAP82, hAKAP82, Fsc1, AKAP4

Clonality: Monoclonal (4G1)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393803

Additional Information

Alternative name: AKAP4 (M10)

Gene ID: 8852

UniProt: NP_003877

Immunogen: AKAP4 (NP_003877, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa

Clone: 4G1

Description: Other names: AKAP82, FSC1, HI, hAKAP82, p82 A kinase (PRKA) anchor protein 4

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AKAP4 (NP_003877, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 94477 DA

Comments:

Background: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

AKAP4 monoclonal antibody (M10), clone 4G1. Western Blot analysis of AKAP4 expression in K-562 ( Cat # L009V1 ).

Publications

Product: Brown, Miki, Harper et al.: "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum." in: Biology of reproduction, Vol. 68, Issue 6, pp. 2241-8, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ross, Grafham, Coffey et al.: "The DNA sequence of the human X chromosome." in: Nature, Vol. 434, Issue 7031, pp. 325-37, 2005 (PubMed).

Lefièvre, Chen, Conner et al.: "Human spermatozoa contain multiple targets for protein S-nitrosylation: an alternative mechanism of the modulation of sperm function by nitric oxide?" in: Proteomics, Vol. 7, Issue 17, pp. 3066-84, 2007 (PubMed).

Moretti, Scapigliati, Pascarelli et al.: "Localization of AKAP4 and tubulin proteins in sperm with reduced motility." in: Asian journal of andrology, Vol. 9, Issue 5, pp. 641-9, 2007 (PubMed).