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Ring Finger Protein 14 (RNF14) (AA 217-317) antibody

Details for Product No. ABIN393809, Supplier: Login to see
Antigen
  • ARA54
  • HFB30
  • TRIAD2
  • 2310075C09Rik
  • 2610005D23Rik
  • AA986456
  • AU041447
  • D18Ertd188e
  • D7Bwg0165e
  • Triad2
Epitope
AA 217-317
20
15
4
3
3
2
2
2
1
1
1
1
Reactivity
Human
68
38
33
1
1
Host
Mouse
66
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
48
24
22
6
6
4
1
1
Supplier
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Immunogen RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4G9
Isotype IgG2a kappa
Specificity RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF14 (RNF14 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 53837 DA
Gene ID 12406
NCBI Accession NP_004281
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Ring Finger Protein 14 (RNF14) (AA 217-317) antibody (ABIN393809) Western blot analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14...
Immunohistochemistry (IHC) image for anti-Ring Finger Protein 14 (RNF14) (AA 217-317) antibody (ABIN393809) anti-Ring Finger Protein 14 (RNF14) (AA 217-317) antibody (Image 2)
Background publications Xu, Shimelis, Linn et al.: "Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination." in: Cancer cell, Vol. 15, Issue 4, pp. 270-82, 2009 (PubMed).

Lan, Hseh, Lu et al.: "Expression of androgen receptor co-regulators in the testes of men with azoospermia." in: Fertility and sterility, Vol. 89, Issue 5 Suppl, pp. 1397-405, 2008 (PubMed).

Kikuchi, Uchida, Hattori et al.: "ARA54 is involved in transcriptional regulation of the cyclin D1 gene in human cancer cells." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1752-8, 2007 (PubMed).

Yang, Chang, Miyamoto et al.: "Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54." in: Endocrinology, Vol. 148, Issue 3, pp. 1340-9, 2007 (PubMed).

Yang, Chang, Miyamoto et al.: "Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 2, pp. 343-58, 2007 (PubMed).