Ring Finger Protein 14 (RNF14) (AA 217-317) antibody

Details for Product No. ABIN393809
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Antigen
Synonyms ARA54, HFB30, TRIAD2, 2310075C09Rik, 2610005D23Rik, AA986456, AU041447, D18Ertd188e, D7Bwg0165e, Triad2
Epitope
AA 217-317
(11), (7), (4), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(55), (38), (33), (25), (25), (24)
Host
Mouse
(54), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB)
(39), (20), (14), (6), (3), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393809
450.00 $
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Immunogen RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4G9
Isotype IgG2a kappa
Specificity RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF14
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 53837 DA
Gene ID 12406
NCBI Accession NP_004281
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Ring Finger Protein 14 (RNF14) (AA 217-317) antibody Western blot analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of RNF14 expression in transfected 293T cell line by RNF14 monoclonal antibody (M01), clone 4G9. Lane 1: RNF14 transfected lysate(39.6 KDa). Lane 2: Non-transfected lysate.
anti-Ring Finger Protein 14 (RNF14) (AA 217-317) antibody (2) anti-Ring Finger Protein 14 (RNF14) (AA 217-317) antibody (Image 2)
Background publications Yang, Chang, Miyamoto et al.: "Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 2, pp. 343-58, 2007 (PubMed).

Yang, Chang, Miyamoto et al.: "Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54." in: Endocrinology, Vol. 148, Issue 3, pp. 1340-9, 2007 (PubMed).

Kikuchi, Uchida, Hattori et al.: "ARA54 is involved in transcriptional regulation of the cyclin D1 gene in human cancer cells." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1752-8, 2007 (PubMed).

Lan, Hseh, Lu et al.: "Expression of androgen receptor co-regulators in the testes of men with azoospermia." in: Fertility and sterility, Vol. 89, Issue 5 Suppl, pp. 1397-405, 2008 (PubMed).

Xu, Shimelis, Linn et al.: "Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination." in: Cancer cell, Vol. 15, Issue 4, pp. 270-82, 2009 (PubMed).

Validation Images
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