|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Ring Finger Protein 14 (RNF14) antibody
|Synonyms||ARA54, HFB30, TRIAD2, FLJ26004, HRIHFB2038|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: ARA54, HFB30, HRIHFB2038 ring finger protein 14|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||53837 DA|
Background: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure|
|Restrictions||For Research Use only|
Yang, Chang, Miyamoto et al.: "Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 2, pp. 343-58, 2007 (PubMed).
Yang, Chang, Miyamoto et al.: "Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54." in: Endocrinology, Vol. 148, Issue 3, pp. 1340-9, 2007 (PubMed).
Kikuchi, Uchida, Hattori et al.: "ARA54 is involved in transcriptional regulation of the cyclin D1 gene in human cancer cells." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1752-8, 2007 (PubMed).
Lan, Hseh, Lu et al.: "Expression of androgen receptor co-regulators in the testes of men with azoospermia." in: Fertility and sterility, Vol. 89, Issue 5 Suppl, pp. 1397-405, 2008 (PubMed).
Xu, Shimelis, Linn et al.: "Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination." in: Cancer cell, Vol. 15, Issue 4, pp. 270-82, 2009 (PubMed).
|Reactivities||Human (46), Rat (Rattus) (41), Mouse (Murine) (37), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Immunofluorescence (IF) (30), Western Blotting (WB) (18), ELISA (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (IHC) (2), Flow Cytometry (FACS) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2)|
|Epitopes||N-Term (3), C-Term (2), C-Term,AA 335-364 (1), Middle Region,Internal Region (1)|