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Details for Product No. ABIN393809

Ring Finger Protein 14 (RNF14) antibody

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Antigen
Synonyms ARA54, HFB30, TRIAD2, 2310075C09Rik, 2610005D23Rik, AA986456, AU041447, D18Ertd188e, D7Bwg0165e, Triad2
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN393809
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4G9
Isotype IgG2a kappa
Specificity RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RNF14
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 53837 DA
Gene ID 12406
UniProt NP_004281
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Yang, Chang, Miyamoto et al.: "Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 2, pp. 343-58, 2007 (PubMed).

Yang, Chang, Miyamoto et al.: "Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54." in: Endocrinology, Vol. 148, Issue 3, pp. 1340-9, 2007 (PubMed).

Kikuchi, Uchida, Hattori et al.: "ARA54 is involved in transcriptional regulation of the cyclin D1 gene in human cancer cells." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1752-8, 2007 (PubMed).

Lan, Hseh, Lu et al.: "Expression of androgen receptor co-regulators in the testes of men with azoospermia." in: Fertility and sterility, Vol. 89, Issue 5 Suppl, pp. 1397-405, 2008 (PubMed).

Xu, Shimelis, Linn et al.: "Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination." in: Cancer cell, Vol. 15, Issue 4, pp. 270-82, 2009 (PubMed).

Alternatives for antigen "Ring Finger Protein 14 (RNF14)", type "Antibodies"
Hosts (45), (3)
Reactivities (46), (35), (30), (25), (25), (24)
Applications (30), (20), (14), (6), (2), (2), (2), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (6), (4), (2), (2), (2), (2), (1), (1)
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