Ring Finger Protein 14 (RNF14) antibody

Antigen: Ring Finger Protein 14 (RNF14)

Synonyms: ARA54, HFB30, TRIAD2, FLJ26004, HRIHFB2038

Clonality: Monoclonal (4G9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393809

Additional Information

Alternative name: RNF14

Gene ID: 9604

UniProt: NP_004281

Immunogen: RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 4G9

Description: Other names: ARA54, HFB30, HRIHFB2038 ring finger protein 14

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RNF14 (NP_004281, 217 a.a. ~ 317 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 53837 DA

Comments:

Background: The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14 Validated Chimera RNAi ( Cat # H00009604-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF14 monoclonal antibody (M01), clone 4G9 (Cat # AT3666a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of RNF14 expression in transfected 293T cell line by RNF14 monoclonal antibody (M01), clone 4G9. Lane 1: RNF14 transfected lysate(39.6 KDa). Lane 2: Non-transfected lysate.

anti-Ring Finger Protein 14 (RNF14) antibody (Image 2)

Publications

Product: Yang, Chang, Miyamoto et al.: "Transgelin functions as a suppressor via inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth." in: Molecular endocrinology (Baltimore, Md.), Vol. 21, Issue 2, pp. 343-58, 2007 (PubMed).

Yang, Chang, Miyamoto et al.: "Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54." in: Endocrinology, Vol. 148, Issue 3, pp. 1340-9, 2007 (PubMed).

Kikuchi, Uchida, Hattori et al.: "ARA54 is involved in transcriptional regulation of the cyclin D1 gene in human cancer cells." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1752-8, 2007 (PubMed).

Lan, Hseh, Lu et al.: "Expression of androgen receptor co-regulators in the testes of men with azoospermia." in: Fertility and sterility, Vol. 89, Issue 5 Suppl, pp. 1397-405, 2008 (PubMed).

Xu, Shimelis, Linn et al.: "Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination." in: Cancer cell, Vol. 15, Issue 4, pp. 270-82, 2009 (PubMed).