Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1632-1731) antibody

Details for Product No. ABIN393812
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Antigen
Synonyms CHD4, im:7143343, si:ch211-51m24.3, si:ch211-57k11.10, wu:fb44b12, wu:fd12d03, MGC145363, Mi-2b, Mi2-BETA, Mi-2, 9530019N15Rik, AA617397, BC005710, D6Ertd380e, Mi-2beta, mKIAA4075, b230399n07
Epitope
AA 1632-1731
(3), (3), (2), (2), (2), (2), (1)
Reactivity
Human
(30), (11), (4), (1), (1), (1), (1)
Host
Mouse
(24), (6)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(19), (17), (13), (7), (6), (6), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.05 mg
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Catalog No. ABIN393812
450.00 $
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Immunogen CHD4 (NP_001264, 1632 a.a. ~ 1731 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 4H4
Isotype IgG2a kappa
Specificity CHD4 (NP_001264, 1632 a.a. ~ 1731 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification Purified
Alternative Name CHD4
Background The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.
Synonyms: Mi2b, Mi2BETA chromodomain helicase DNA binding protein 4
Molecular Weight 217991 DA
Gene ID 1108
NCBI Accession NP_001264
Research Area Signaling, Protein Modifications, Transcription Factors, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

Restrictions For Research Use only
Buffer Phosphate buffered saline, pH 7.2
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1632-1731) antibody Western Blot analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4. Lane 1: CHD4 transfected lysate(220 KDa). Lane 2: Non-transfected lysate. CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in HeLa NE.
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1632-1731) antibody (2) anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1632-1731) antibody (Image 2)
Background publications Ishii, Du, Zhang et al.: "Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7533-41, 2009 (PubMed).

Helbling Chadwick, Chadwick, Jaye et al.: "The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells." in: Chromosoma, Vol. 118, Issue 4, pp. 445-57, 2009 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Musselman, Mansfield, Garske et al.: "Binding of the CHD4 PHD2 finger to histone H3 is modulated by covalent modifications." in: The Biochemical journal, Vol. 423, Issue 2, pp. 179-87, 2009 (PubMed).

Li, Zhang, Yu et al.: "ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis." in: The EMBO journal, Vol. 28, Issue 18, pp. 2763-76, 2009 (PubMed).

Validation Images
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