SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody

Details for Product No. ABIN393845
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Antigen
Synonyms d11moh34, vps22, Dot3, EAP30, VPS22, D11moh34, RGD1310144, D11Moh34, D11MOH34, zgc:101578
Epitope
AA 23-123
(16), (7), (7), (5), (2), (2), (2), (2), (2), (1), (1)
Reactivity
Human
(41), (13), (10), (4), (4), (4), (4), (2), (1), (1)
Host
Mouse
(31), (10), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(41), (18), (8), (3), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393845
450.00 $
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Immunogen EAP30 (NP_009172, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6B11
Isotype IgG1 kappa
Specificity EAP30 (NP_009172, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EAP30
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 28864 DA
Gene ID 12406
NCBI Accession NP_009172
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL, MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741], Kamura et al., 2001 [PubMed 11278625]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody EAP30 monoclonal antibody (M01), clone 6B11 Western Blot analysis of SNF8 expression in HeLa NE. Detection limit for recombinant GST tagged SNF8 is approximately 1 ng/mL as a capture antibody.
anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody (2) anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody (Image 2)
Background publications Slagsvold, Aasland, Hirano et al.: "Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19600-6, 2005 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Bowers, Piper, Edeling et al.: "Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 5094-105, 2006 (PubMed).

Langelier, von Schwedler, Fisher et al.: "Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release." in: Journal of virology, Vol. 80, Issue 19, pp. 9465-80, 2006 (PubMed).

Malerød, Stuffers, Brech et al.: "Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 11, pp. 1617-29, 2007 (PubMed).

Validation Images
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