SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) antibody

Antigen: SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8)

Synonyms: Dot3, EAP30, VPS22, D11moh34, MGC105799, RGD1310144, D11MOH34, d11moh34, SNF8, MGC142498, MGC7335, D11Moh34, RP23-235J5.2, zgc:101578, vps22

Clonality: Monoclonal (6B11)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393845

Additional Information

Alternative name: EAP30

Gene ID: 11267

UniProt: NP_009172

Immunogen: EAP30 (NP_009172, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 6B11

Description: Other names: EAP30 subunit of ELL complex

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EAP30 (NP_009172, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 28864 DA

Comments:

Background: SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL, MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741], Kamura et al., 2001 [PubMed 11278625]).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

EAP30 monoclonal antibody (M01), clone 6B11 Western Blot analysis of SNF8 expression in HeLa NE ( Cat # L013V3 ). Detection limit for recombinant GST tagged SNF8 is approximately 1ng/ml as a capture antibody.

anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) antibody (Image 2)

Publications

Product: Slagsvold, Aasland, Hirano et al.: "Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19600-6, 2005 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Bowers, Piper, Edeling et al.: "Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 5094-105, 2006 (PubMed).

Langelier, von Schwedler, Fisher et al.: "Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release." in: Journal of virology, Vol. 80, Issue 19, pp. 9465-80, 2006 (PubMed).

Malerød, Stuffers, Brech et al.: "Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 11, pp. 1617-29, 2007 (PubMed).