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SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody

Details for Product No. ABIN393845, Supplier: Log in to see
Antigen
  • d11moh34
  • vps22
  • Dot3
  • EAP30
  • VPS22
  • D11moh34
  • RGD1310144
  • D11Moh34
  • D11MOH34
  • zgc:101578
Epitope
AA 23-123
25
15
5
3
2
2
2
2
2
1
1
Reactivity
Human
47
15
12
4
4
4
4
2
1
Host
Mouse
41
5
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
44
23
6
2
1
1
Supplier
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Immunogen EAP30 (NP_009172, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6B11
Isotype IgG1 kappa
Specificity EAP30 (NP_009172, 23 a.a. ~ 123 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EAP30 (SNF8 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 28864 DA
Gene ID 12406
NCBI Accession NP_009172
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL, MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741], Kamura et al., 2001 [PubMed 11278625]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody (ABIN393845) EAP30 monoclonal antibody (M01), clone 6B11 Western Blot analysis of SNF8 expression ...
Immunohistochemistry (IHC) image for anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody (ABIN393845) anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (AA 23-123) antibody (Image 2)
Background publications Malerød, Stuffers, Brech et al.: "Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 11, pp. 1617-29, 2007 (PubMed).

Langelier, von Schwedler, Fisher et al.: "Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release." in: Journal of virology, Vol. 80, Issue 19, pp. 9465-80, 2006 (PubMed).

Bowers, Piper, Edeling et al.: "Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 5094-105, 2006 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Slagsvold, Aasland, Hirano et al.: "Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19600-6, 2005 (PubMed).