Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) antibody

Details for Product No. ABIN393852
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Synonyms MAPKKK4, MEKK4, MTK1, PRO0412, D17Rp17, D17Rp17e, MEKK 4, Mek4b, Mekk4, RP17, Rp17a, Tas, mKIAA0213
(38), (26), (14), (12), (12), (12), (12)
(27), (11)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(21), (18), (10), (10), (10), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN393852
Quantity 0.1 mg
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Immunogen MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6C6
Isotype IgG1 kappa
Specificity MAP3K4 (NP_005913, 1201 a.a. ~ 1300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAP3K4
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 181552 DA
Gene ID 12406
UniProt NP_005913
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000

Background: The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) antibody MAP3K4 monoclonal antibody (M02), clone 6C6 Western Blot analysis of MAP3K4 expression in NIH/3T3 on formalin-fixed paraffin-embedded human testis (antibody concentration 3 µg/mL).
anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) antibody (2) anti-Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) antibody (Image 2)
Background publications Derbyshire, Halfter, Heimark et al.: "Angiotensin II stimulated transcription of cyclooxygenase II is regulated by a novel kinase cascade involving Pyk2, MEKK4 and annexin II." in: Molecular and cellular biochemistry, Vol. 271, Issue 1-2, pp. 77-90, 2005 (PubMed).

Abell, Johnson: "MEKK4 is an effector of the embryonic TRAF4 for JNK activation." in: The Journal of biological chemistry, Vol. 280, Issue 43, pp. 35793-6, 2005 (PubMed).

Aissouni, Zapart, Iovanna et al.: "CIN85 regulates the ability of MEKK4 to activate the p38 MAP kinase pathway." in: Biochemical and biophysical research communications, Vol. 338, Issue 2, pp. 808-14, 2005 (PubMed).

Whitmarsh, Davis: "Role of mitogen-activated protein kinase kinase 4 in cancer." in: Oncogene, Vol. 26, Issue 22, pp. 3172-84, 2007 (PubMed).

Grucza, Johnson, Krueger et al.: "Incorporating age at onset of smoking into genetic models for nicotine dependence: evidence for interaction with multiple genes." in: Addiction biology, Vol. 15, Issue 3, pp. 346-57, 2010 (PubMed).

Hosts (27), (11)
Reactivities (38), (26), (14), (12), (12), (12), (12)
Applications (21), (18), (10), (10), (10), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (2), (1), (1)
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