F-Box Protein 22 (FBXO22) (AA 1-300) antibody

Details for Product No. ABIN393870, Supplier: Log in to see
Antigen
  • FBXO22
  • DKFZp469L0535
  • fbxo22
  • MGC146708
  • si:busm1-132m23.3
  • FBX22
  • FISTC1
  • 0610033L19Rik
  • 1600016C16Rik
  • F-box protein 22
  • F-box only protein 22
  • FBXO22
  • fbxo22
  • LOC100342921
  • Fbxo22
Alternatives
anti-Human F-Box Protein 22 antibody for ELISA
Epitope
AA 1-300
10
9
9
4
1
1
1
1
1
1
Reactivity
Human
38
24
7
4
4
3
2
1
1
1
Host
Mouse
37
10
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
44
29
3
Options
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Immunogen FBXO22 (AAH39024, 1 a.a. ~ 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6G9
Isotype IgG1 kappa
Specificity FBXO22 (AAH39024, 1 a.a. ~ 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name FBXO22 (FBXO22 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 44508 DA
Gene ID 12406
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Two transcript variants encoding different isoforms exist for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-F-Box Protein 22 (FBXO22) (AA 1-300) antibody (ABIN393870) FBXO22 monoclonal antibody (M01), clone 6G9 Western Blot analysis of FBXO22 expressio...
Immunohistochemistry (IHC) image for anti-F-Box Protein 22 (FBXO22) (AA 1-300) antibody (ABIN393870) anti-F-Box Protein 22 (FBXO22) (AA 1-300) antibody (Image 2)
Background publications Sowa, Bennett, Gygi, Harper: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Borziak, Zhulin: "FIST: a sensory domain for diverse signal transduction pathways in prokaryotes and ubiquitin signaling in eukaryotes." in: Bioinformatics (Oxford, England), Vol. 23, Issue 19, pp. 2518-21, 2007 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Dias, Dolios, Wang, Pan: "CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16601-6, 2002 (PubMed).