F-Box Protein 22 (FBXO22) antibody

Antigen: F-Box Protein 22 (FBXO22)

Synonyms: FBX22, FISTC1, FLJ13986, MGC31799, 0610033L19Rik, 1600016C16Rik, MGC124731, FBXO22, MGC157370, DKFZp469L0535, fbxo22, MGC146708

Clonality: Monoclonal (6G9)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN393870

Additional Information

Alternative name: FBXO22

Gene ID: 26263

Immunogen: FBXO22 (AAH39024, 1 a.a. ~ 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 6G9

Description: Other names: FBX22, FLJ13986 F-box protein 22

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FBXO22 (AAH39024, 1 a.a. ~ 300 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 44508 DA

Comments:

Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Two transcript variants encoding different isoforms exist for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

FBXO22 monoclonal antibody (M01), clone 6G9 Western Blot analysis of FBXO22 expression in Hela NE ( Cat # L013V3 ). Detection limit for recombinant GST tagged FBXO22 is approximately 0.3ng/ml as a capture antibody.

anti-F-Box Protein 22 (FBXO22) antibody (Image 2)

Publications

Product: Dias, Dolios, Wang et al.: "CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16601-6, 2002 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Borziak, Zhulin: "FIST: a sensory domain for diverse signal transduction pathways in prokaryotes and ubiquitin signaling in eukaryotes." in: Bioinformatics (Oxford, England), Vol. 23, Issue 19, pp. 2518-21, 2007 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).