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Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody

Details for Product No. ABIN393872, Supplier: Log in to see
Antigen
  • DCLK1
  • DCAMKL1
  • dcamkl1
  • dclk1
  • wu:fc51f11
  • zgc:153709
  • MGC145348
  • CL1
  • CLICK1
  • DCDC3A
  • DCLK
  • 1700113D08Rik
  • 2810480F11Rik
  • AI836758
  • Click-I
  • Cpg16
  • Dcamkl1
  • Dcl
  • Dclk
  • mKIAA0369
  • Ania4
Epitope
AA 640-729
45
24
15
14
7
5
3
3
2
2
2
1
1
Reactivity
Human
89
58
28
1
1
1
1
1
1
Host
Mouse
99
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
87
65
23
15
11
7
7
3
1
Supplier
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Immunogen DCAMKL1 (NP_004725, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6H4
Isotype IgG Mix kappa
Specificity DCAMKL1 (NP_004725, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DCAMKL1 (DCLK1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 82224 DA
Gene ID 12406
NCBI Accession NP_004725
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody (ABIN393872) Immunofluorescence of monoclonal antibody to DCAMKL1 on NIH/3T3 cell (antibody concen...
Immunohistochemistry (IHC) image for anti-Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody (ABIN393872) anti-Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody (Image 2)
Background publications Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Dijkmans, van Hooijdonk, Fitzsimons et al.: "The doublecortin gene family and disorders of neuronal structure." in: Central nervous system agents in medicinal chemistry, Vol. 10, Issue 1, pp. 32-46, 2010 (PubMed).

Verissimo, Molenaar, Meerman et al.: "Silencing of the microtubule-associated proteins doublecortin-like and doublecortin-like kinase-long induces apoptosis in neuroblastoma cells." in: Endocrine-related cancer, Vol. 17, Issue 2, pp. 399-414, 2010 (PubMed).

Le Hellard, Håvik, Espeseth et al.: "Variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, are associated with memory and general cognitive abilities." in: PLoS ONE, Vol. 4, Issue 10, pp. e7534, 2009 (PubMed).

Schenk, Engels, Zhang et al.: "A potential role for calcium / calmodulin-dependent protein kinase-related peptide in neuronal apoptosis: in vivo and in vitro evidence." in: The European journal of neuroscience, Vol. 26, Issue 12, pp. 3411-20, 2007 (PubMed).