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Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody

Details for Product No. ABIN393872
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Antigen
Synonyms DCLK1, DCAMKL1, dcamkl1, dclk1, wu:fc51f11, zgc:153709, MGC145348, CL1, CLICK1, DCDC3A, DCLK, 1700113D08Rik, 2810480F11Rik, AI836758, Click-I, Cpg16, Dcamkl1, Dcl, Dclk, mKIAA0369, Ania4
Epitope
AA 640-729
(25), (13), (7), (6), (6), (3), (2), (2), (1), (1), (1)
Reactivity
Human
(57), (37), (23), (12), (12), (12)
Host
Mouse
(59), (6), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(57), (38), (15), (10), (6), (6), (5), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN393872
450.00 $
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Immunogen DCAMKL1 (NP_004725, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 6H4
Isotype IgG Mix kappa
Specificity DCAMKL1 (NP_004725, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DCAMKL1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 82224 DA
Gene ID 12406
UniProt NP_004725
Research Area Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. The encoded protein is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. This gene is up-regulated by brain-derived neurotrophic factor and associated with memory and general cognitive abilities. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported, but the full-length nature and biological validity of some variants have not been defined. These variants encode different isoforms, which are differentially expressed and have different kinase activities.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody Immunofluorescence of monoclonal antibody to DCAMKL1 on NIH/3T3 cell (antibody concentration 10 µg/mL). DCAMKL1 monoclonal antibody (M03), clone 6H4 Western Blot analysis of DCAMKL1 expression in NIH/3T3.
anti-Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody (2) anti-Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) antibody (Image 2)
Background publications Schenk, Engels, Zhang et al.: "A potential role for calcium / calmodulin-dependent protein kinase-related peptide in neuronal apoptosis: in vivo and in vitro evidence." in: The European journal of neuroscience, Vol. 26, Issue 12, pp. 3411-20, 2007 (PubMed).

Le Hellard, Håvik, Espeseth et al.: "Variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, are associated with memory and general cognitive abilities." in: PLoS ONE, Vol. 4, Issue 10, pp. e7534, 2009 (PubMed).

Verissimo, Molenaar, Meerman et al.: "Silencing of the microtubule-associated proteins doublecortin-like and doublecortin-like kinase-long induces apoptosis in neuroblastoma cells." in: Endocrine-related cancer, Vol. 17, Issue 2, pp. 399-414, 2010 (PubMed).

Dijkmans, van Hooijdonk, Fitzsimons et al.: "The doublecortin gene family and disorders of neuronal structure." in: Central nervous system agents in medicinal chemistry, Vol. 10, Issue 1, pp. 32-46, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Validation Images
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