|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Acetyl-CoA Carboxylase alpha (ACACA) antibody
|Synonyms||ACC, ACAC, ACC1, ACCA|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ACACA (NP_942131, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: ACAC, ACC, ACC1, ACCA acetyl-Coenzyme A carboxylase alpha|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ACACA (NP_942131, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||265554 DA|
Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged ACACA is approximately 0.03ng/ml as a capture antibody.|
Zhao, Iwasaki, Zhe et al.: "Hormonal regulation of acetyl-CoA carboxylase isoenzyme gene transcription." in: Endocrine journal, Vol. 57, Issue 4, pp. 317-24, 2010 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).
Kim, Moon, Park et al.: "Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 21, pp. 9626-31, 2010 (PubMed).
Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).
Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).
|Hosts||Rabbit (59), Mouse (1)|
|Reactivities||Human (57), Rat (Rattus) (44), Mouse (Murine) (38), Cow (Bovine) (36), Dog (Canine) (36), Horse (Equine) (36), Pig (Porcine) (36), Sheep (Ovine) (36), Guinea Pig (18), Rabbit (18)|
|Applications||Immunofluorescence (IF) (33), Western Blotting (WB) (30), ELISA (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Immunohistochemistry (IHC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoprecipitation (IP) (5), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (2), Immunocytochemistry (ICC) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2)|
|Epitopes||pSer79 (22), N-Term (4), pS1263 (2), pSer1263 (2), AA 1925-1975 (1), C-Term (1), Lys557 (1), pSer523 (1), pSer80 (1)|